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Alternative name
Programmed cell death 1 ligand 1 ELISA KIT; B7 homolog 1 ELISA KIT; B7-H1 ELISA KIT; CD_antigen: CD274CD274 ELISA KIT; B7H1 ELISA KIT; PDCD1L1 ELISA KIT; PDCD1LG1 ELISA KIT; PDL1 ELISA KIT; PD-L1 ELISA KIT; PDCD1 ligand 1 ELISA KIT; Programmed death ligand 1 ELISA KIT; B7-H1 ELISA KIT
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Catalog
E064664
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species
Mouse
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GenePDL1/B7-H1/CD274
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SpecificityThis assay has high sensitivity and excellent specificity for detection of PDL1/B7-H1/CD274. No significant cross-reactivity or interference between PDL1/B7-H1/CD274 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PDL1/B7-H1/CD274 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseMouse PDL1/B7-H1/CD274 ELISA Kit allows for the in vitro quantitative determination of PDL1/B7-H1/CD274 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: PDL1/B7-H1/CD274 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDL1/B7-H1/CD274 antibody and an PDL1/B7-H1/CD274-HRP conjugate. The assay sample and buffer are incubated together with PDL1/B7-H1/CD274-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDL1/B7-H1/CD274 concentration since PDL1/B7-H1/CD274 from samples and PDL1/B7-H1/CD274-HRP conjugate compete for the anti-PDL1/B7-H1/CD274 antibody binding site. Since the number of sites is limited, as more sites are occupied by PDL1/B7-H1/CD274 from the sample, fewer sites are left to bind PDL1/B7-H1/CD274-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDL1/B7-H1/CD274 concentration in each sample is interpolated from this standard curve.
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