Human MET ELISA Kit

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  • Alternative name

    Human HGF receptor ELISA Kit;Human HGF/SF receptor ELISA Kit;Human proto-oncogene c-Met ELISA Kit;Human scatter factor receptor ELISA Kit;Human SF receptor ELISA Kit;Human tyrosine-protein kinase Met ELISA Kit;Human AUTS9 ELISA Kit;Human DFNB97 ELISA Kit;Human HGFR ELISA Kit;Human RCCP2 ELISA Kit;Human c-Met ELISA Kit;Human MET proto-oncogene, receptor tyrosine kinase ELISA Kit;Human hepatocyte growth factor receptor ELISA Kit;

  • Catalog
    E005573
  • species
    Human
  • GeneMET
  • Standard CurveHuman MET ELISA Kit
  • Other Species Human c-MET/HGFR ELISA KitHuman C.met ELISA KitHuman c-MET ELISA KitHuman Met ENK ELISA KitMouse C-MET ELISA KitMouse Met ELISA KitMouse C.met ELISA KitMouse Met-ENK ELISA KitBovine MET ELISA KitCanine MET ELISA KitHorse MET ELISA KitPorcine MET ELISA KitRabbit MET ELISA KitRat Met ELISA KitSheep MET ELISA Kit
  • SpecificityNatural and recombinant human C-MET
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 5pg/ml
  • Intended UseHuman MET ELISA Kit allows for the in vitro quantitative determination of MET , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human C-MET ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for C-MET has been precoated onto 96-well plates. Standards(NSO,E25 - R307 (alpha) &S308 - T932(beta)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for C-MET is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human C-MET amount of sample captured in plate. Background: C-Met (MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.
  • Human Hepatocyte growth factor receptor Protein information
  • Uniprot ID MET_HUMAN
  • Uniprot AC P08581; A1L467; B5A932; E7EQ94; O60366; Q12875; Q9UDX7; Q9UPL8;
  • UniGene Hs.132966;
  • GeneID 4233
  • KEGG hsa:4233;
  • Human Hepatocyte growth factor receptor Protein SEQUENCE
  • SEQUENCE 1390 AA; 155541 MW; 9CF896D1273905C3 CRC64;

    MKAPAVLAPG ILVLLFTLVQ RSNGECKEAL AKSEMNVNMK YQLPNFTAET

    PIQNVILHEH HIFLGATNYI YVLNEEDLQK VAEYKTGPVL EHPDCFPCQD

    CSSKANLSGG VWKDNINMAL VVDTYYDDQL ISCGSVNRGT CQRHVFPHNH

    TADIQSEVHC IFSPQIEEPS QCPDCVVSAL GAKVLSSVKD RFINFFVGNT

    INSSYFPDHP LHSISVRRLK ETKDGFMFLT DQSYIDVLPE FRDSYPIKYV

    HAFESNNFIY FLTVQRETLD AQTFHTRIIR FCSINSGLHS YMEMPLECIL

    TEKRKKRSTK KEVFNILQAA YVSKPGAQLA RQIGASLNDD ILFGVFAQSK

    PDSAEPMDRS AMCAFPIKYV NDFFNKIVNK NNVRCLQHFY GPNHEHCFNR

    TLLRNSSGCE ARRDEYRTEF TTALQRVDLF MGQFSEVLLT SISTFIKGDL

    TIANLGTSEG RFMQVVVSRS GPSTPHVNFL LDSHPVSPEV IVEHTLNQNG

    YTLVITGKKI TKIPLNGLGC RHFQSCSQCL SAPPFVQCGW CHDKCVRSEE

    CLSGTWTQQI CLPAIYKVFP NSAPLEGGTR LTICGWDFGF RRNNKFDLKK

    TRVLLGNESC TLTLSESTMN TLKCTVGPAM NKHFNMSIII SNGHGTTQYS

    TFSYVDPVIT SISPKYGPMA GGTLLTLTGN YLNSGNSRHI SIGGKTCTLK

    SVSNSILECY TPAQTISTEF AVKLKIDLAN RETSIFSYRE DPIVYEIHPT

    KSFISGGSTI TGVGKNLNSV SVPRMVINVH EAGRNFTVAC QHRSNSEIIC

    CTTPSLQQLN LQLPLKTKAF FMLDGILSKY FDLIYVHNPV FKPFEKPVMI

    SMGNENVLEI KGNDIDPEAV KGEVLKVGNK SCENIHLHSE AVLCTVPNDL

    LKLNSELNIE WKQAISSTVL GKVIVQPDQN FTGLIAGVVS ISTALLLLLG

    FFLWLKKRKQ IKDLGSELVR YDARVHTPHL DRLVSARSVS PTTEMVSNES

    VDYRATFPED QFPNSSQNGS CRQVQYPLTD MSPILTSGDS DISSPLLQNT

    VHIDLSALNP ELVQAVQHVV IGPSSLIVHF NEVIGRGHFG CVYHGTLLDN

    DGKKIHCAVK SLNRITDIGE VSQFLTEGII MKDFSHPNVL SLLGICLRSE

    GSPLVVLPYM KHGDLRNFIR NETHNPTVKD LIGFGLQVAK GMKYLASKKF

    VHRDLAARNC MLDEKFTVKV ADFGLARDMY DKEYYSVHNK TGAKLPVKWM

    ALESLQTQKF TTKSDVWSFG VLLWELMTRG APPYPDVNTF DITVYLLQGR

    RLLQPEYCPD PLYEVMLKCW HPKAEMRPSF SELVSRISAI FSTFIGEHYV

    HVNATYVNVK CVAPYPSLLS SEDNADDEVD TRPASFWETS

  • UCSC uc003vij.4; human. [P08581-1];




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