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Mouse Hcn4 ELISA Kit

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  • Alternative name

    Mouse Brain cyclic nucleotide-gated channel 3 ELISA Kit;Mouse BCNG-3 ELISA Kit;Mouse Bcng3 ELISA Kit;
  • Catalog
    E064377
  • species
    Mouse
  • GeneHcn4
  • SpecificityThis assay recognizes recombinant and natural mouse Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Hcn4 ELISA Kit allows for the in vitro quantitative determination of Hcn4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Protein information
  • Uniprot ID HCN4_MOUSE
  • Uniprot AC O70507;
  • UniGene Mm.151967;
  • Mouse Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 Protein SEQUENCE

  • SEQUENCE 1186 AA; 127414 MW; B3DC16818E8697DC CRC64;

    MDKLPPSMRK RLYSLPQQVG AKAWIMDEEE DGEEEGAGGR QDPSRRSIRL

    RPLPSPSPSV AAGCSESRGA ALGATESEGP GRSAGKSSTN GDCRRFRGSL

    ASLGSRGGGS GGAGGGSSLG HLHDSAEERR LIAAEGDASP GEDRTPPGLA

    TEPERPATAA QPAASPPPQQ PPQPASASCE QPSADTAIKV EGGAAAIDHI

    LPEAEVRLGQ SGFMQRQFGA MLQPGVNKFS LRMFGSQKAV EREQERVKSA

    GFWIIHPYSD FRFYWDLTML LLMVGNLIII PVGITFFKDE NTTPWIVFNV

    VSDTFFLIDL VLNFRTGIVV EDNTEIILDP QRIKMKYLKS WFVVDFISSI

    PVEYIFLIVE TRIDSEVYKT ARAVRIVRFT KILSLLRLLR LSRLIRYIHQ

    WEEIFHMTYD LASAVVRIVN LIGMMLLLCH WDGCLQFLVP MLQDFPHDCW

    VSINGMVNNS WGKQYSYALF KAMSHMLCIG YGRQAPVGMS DVWLTMLSMI

    VGATCYAMFI GHATALIQSL DSSRRQYQEK YKQVEQYMSF HKLPPDTRQR

    IHDYYEHRYQ GKMFDEESIL GELSEPLREE IINFNCRKLV ASMPLFANAD

    PNFVTSMLTK LRFEVFQPGD YIIREGTIGK KMYFIQHGVV SVLTKGNKET

    RLADGSYFGE ICLLTRGRRT ASVRADTYCR LYSLSVDNFN EVLEEYPMMR

    KKNSILLHKV QHDLNSGVFN YQENEIIQQI VRHDREMAHC AHRVQAAASA

    TPTPTPVIWT PLIQAPLQAA AATTSVAIAL THHPRLPAAI FRPPPGPGLG

    NLGAGQTPRH PRRLQSLIPS ALGSASPASS PSQVDTPSSS SFHIQQLAGF

    SAPPGLSPLL PSSSSSPPPG ACGSPPAPTP STSTAAAAST TGFGHFHKAL

    GGSLSSSDSP LLTPLQPGAR SPQAAQPPPP LPGARGGLGL LEHFLPPPPS

    SRSPSSSPGQ LGQPPGELSL GLAAGPSSTP ETPPRPERPS FMAGASGGAS

    PVAFTPRGGL SPPGHSPGPP RTFPSAPPRA SGSHGSLLLP PASSPPPPQV

    PQRRGTPPLT PGRLTQDLKL ISASQPALPQ DGAQTLRRAS PHSSGESVAA

    FSLYPRAGGG SGSSGGLGPP GRPYGAIPGQ HVTLPRKTSS GSLPPPLSLF

    GARAASSGGP PLTTAAPQRE PGARSEPVRS KLPSNL




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