Human MAN2B2 ELISA Kit

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  • Alternative name

    Human mannosidase alpha class 2B member 2 ELISA Kit;Human KIAA0935 ELISA Kit;Human epididymis-specific alpha-mannosidase ELISA Kit;Human core-specific lysosomal alpha-1,6-Mannosidase ELISA Kit;

  • Catalog
    E013417
  • species
    Human
  • GeneMAN2B2
  • Standard CurveHuman MAN2B2 ELISA Kit
  • Other Species Mouse Man2b2 ELISA KitPorcine MAN2B2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MAN2B2. No significant cross-reactivity or interference between MAN2B2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MAN2B2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman MAN2B2 ELISA Kit allows for the in vitro quantitative determination of MAN2B2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: MAN2B2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAN2B2 antibody and an MAN2B2-HRP conjugate. The assay sample and buffer are incubated together with MAN2B2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAN2B2 concentration since MAN2B2 from samples and MAN2B2-HRP conjugate compete for the anti-MAN2B2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MAN2B2 from the sample, fewer sites are left to bind MAN2B2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAN2B2 concentration in each sample is interpolated from this standard curve.
  • Human Epididymis-specific alpha-mannosidase Protein information
  • Uniprot ID MA2B2_HUMAN
  • Uniprot AC Q9Y2E5; Q66MP2; Q86T67;
  • UniGene Hs.188464;
  • GeneID 23324
  • KEGG hsa:23324;
  • Human Epididymis-specific alpha-mannosidase Protein SEQUENCE
  • SEQUENCE 1009 AA; 113979 MW; 21BB879400E0F387 CRC64;

    MGQLCWLPLL APLLLLRPPG VQSAGPIRAF VVPHSHMDVG WVYTVQESMR

    AYAANVYTSV VEELARGQQR RFIAVEQEFF RLWWDGVASD QQKYQVRQLL

    EEGRLEFVIG GQVMHDEAVT HLDDQILQLT EGHGFLYETF GIRPQFSWHV

    DPFGASATTP TLFALAGFNA HLGSRIDYDL KAAMQEARGL QFVWRGSPSL

    SERQEIFTHI MDQYSYCTPS HIPFSNRSGF YWNGVAVFPK PPQDGVYPNM

    SEPVTPANIN LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFA

    NMDPLLDHIN SHAAELGVSV QYATLGDYFR ALHALNVTWR VRDHHDFLPY

    STEPFQAWTG FYTSRSSLKG LARRASALLY AGESMFTRYL WPAPRGHLDP

    TWALQQLQQL RWAVSEVQHH DAITGTESPK VRDMYATHLA SGMLGMRKLM

    ASIVLDELQP QAPMAASSDA GPAGHFASVY NPLAWTVTTI VTLTVGFPGV

    RVTDEAGHPV PSQIQNSTET PSAYDLLILT TIPGLSYRHY NIRPTAGAQE

    GTQEPAATVA STLQFGRRLR RRTSHAGRYL VPVANDCYIV LLDQDTNLMH

    SIWERQSNRT VRVTQEFLEY HVNGDVKQGP ISDNYLFTPG KAAVPAWEAV

    EMEIVAGQLV TEIRQYFYRN MTAQNYTYAI RSRLTHVPQG HDGELLCHRI

    EQEYQAGPLE LNREAVLRTS TNLNSQQVIY SDNNGYQMQR RPYVSYVNNS

    IARNYYPMVQ SAFMEDGKSR LVLLSERAHG ISSQGNGQVE VMLHRRLWNN

    FDWDLGYNLT LNDTSVVHPV LWLLLGSWSL TTALRQRSAL ALQHRPVVLF

    GDLAGTAPKL PGPQQQEAVT LPPNLHLQIL SIPGWRYSSN HTEHSQNLRK

    GHRGEAQADL RRVLLRLYHL YEVGEDPVLS QPVTVNLEAV LQALGSVVAV

    EERSLTGTWD LSMLHRWSWR TGPGRHRGDT TSPSRPPGGP IITVHPKEIR

    TFFIHFQQQ

  • UCSC uc003gjf.2; human. [Q9Y2E5-1];


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