Human MAN2B2 ELISA Kit

Alternative name
Human mannosidase alpha class 2B member 2 ELISA Kit;Human KIAA0935 ELISA Kit;Human epididymis-specific alpha-mannosidase ELISA Kit;Human core-specific lysosomal alpha-1,6-Mannosidase ELISA Kit;
Catalog
E013417
species
Human
GeneMAN2B2
Standard Curve
Other Species Mouse Man2b2 ELISA Kit Porcine MAN2B2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of MAN2B2. No significant cross-reactivity or interference between MAN2B2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MAN2B2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman MAN2B2 ELISA Kit allows for the in vitro quantitative determination of MAN2B2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: MAN2B2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAN2B2 antibody and an MAN2B2-HRP conjugate. The assay sample and buffer are incubated together with MAN2B2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAN2B2 concentration since MAN2B2 from samples and MAN2B2-HRP conjugate compete for the anti-MAN2B2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MAN2B2 from the sample, fewer sites are left to bind MAN2B2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAN2B2 concentration in each sample is interpolated from this standard curve.
Human Epididymis-specific alpha-mannosidase Protein information
Uniprot ID MA2B2_HUMAN
Uniprot AC Q9Y2E5; Q66MP2; Q86T67;
UniGene Hs.188464;
GeneID 23324
KEGG hsa:23324;
Human Epididymis-specific alpha-mannosidase Protein SEQUENCE
SEQUENCE 1009 AA; 113979 MW; 21BB879400E0F387 CRC64;
MGQLCWLPLL APLLLLRPPG VQSAGPIRAF VVPHSHMDVG WVYTVQESMR
AYAANVYTSV VEELARGQQR RFIAVEQEFF RLWWDGVASD QQKYQVRQLL
EEGRLEFVIG GQVMHDEAVT HLDDQILQLT EGHGFLYETF GIRPQFSWHV
DPFGASATTP TLFALAGFNA HLGSRIDYDL KAAMQEARGL QFVWRGSPSL
SERQEIFTHI MDQYSYCTPS HIPFSNRSGF YWNGVAVFPK PPQDGVYPNM
SEPVTPANIN LYAEALVANV KQRAAWFRTP HVLWPWGCDK QFFNASVQFA
NMDPLLDHIN SHAAELGVSV QYATLGDYFR ALHALNVTWR VRDHHDFLPY
STEPFQAWTG FYTSRSSLKG LARRASALLY AGESMFTRYL WPAPRGHLDP
TWALQQLQQL RWAVSEVQHH DAITGTESPK VRDMYATHLA SGMLGMRKLM
ASIVLDELQP QAPMAASSDA GPAGHFASVY NPLAWTVTTI VTLTVGFPGV
RVTDEAGHPV PSQIQNSTET PSAYDLLILT TIPGLSYRHY NIRPTAGAQE
GTQEPAATVA STLQFGRRLR RRTSHAGRYL VPVANDCYIV LLDQDTNLMH
SIWERQSNRT VRVTQEFLEY HVNGDVKQGP ISDNYLFTPG KAAVPAWEAV
EMEIVAGQLV TEIRQYFYRN MTAQNYTYAI RSRLTHVPQG HDGELLCHRI
EQEYQAGPLE LNREAVLRTS TNLNSQQVIY SDNNGYQMQR RPYVSYVNNS
IARNYYPMVQ SAFMEDGKSR LVLLSERAHG ISSQGNGQVE VMLHRRLWNN
FDWDLGYNLT LNDTSVVHPV LWLLLGSWSL TTALRQRSAL ALQHRPVVLF
GDLAGTAPKL PGPQQQEAVT LPPNLHLQIL SIPGWRYSSN HTEHSQNLRK
GHRGEAQADL RRVLLRLYHL YEVGEDPVLS QPVTVNLEAV LQALGSVVAV
EERSLTGTWD LSMLHRWSWR TGPGRHRGDT TSPSRPPGGP IITVHPKEIR
TFFIHFQQQ
UCSC uc003gjf.2; human. [Q9Y2E5-1];

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anti-MAN2B2 antibody

Category

Human MAN2B2 ELISA Kit

Human mannosidase alpha class 2B member 2 ELISA Kit;Human KIAA0935 ELISA Kit;Human epididymis-specific alpha-mannosidase ELISA Kit;Human core-specific lysosomal alpha-1,6-Mannosidase ELISA Kit;