Human PIGF ELISA Kit

Alternative name
Human PIG-F ELISA Kit;Human GPI11 homolog ELISA Kit;Human phosphatidylinositol glycan anchor biosynthesis class F ELISA Kit;Human phosphatidylinositol-glycan biosynthesis class F protein ELISA Kit;
Catalog
E028941
species
Human
GenePIGF
Other Species Mouse Pigf ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PIGF. No significant cross-reactivity or interference between human PIGF and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PIGF is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman PIGF ELISA Kit allows for the in vitro quantitative determination of PIGF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPriciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PIGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Phosphatidylinositol-glycan biosynthesis class F protein Protein information
Uniprot ID PIGF_HUMAN
Uniprot AC Q07326; Q8WW20;
UniGene Hs.468415;
GeneID 5281
KEGG hsa:5281;
Human Phosphatidylinositol-glycan biosynthesis class F protein Protein SEQUENCE
SEQUENCE 219 AA; 24890 MW; 313F97A55B781EBF CRC64;
MKDNDIKRLL YTHLLCIFSI ILSVFIPSLF LENFSILETH LTWLCICSGF
VTAVNLVLYL VVKPNTSSKR SSLSHKVTGF LKCCIYFLMS CFSFHVIFVL
YGAPLIELAL ETFLFAVILS TFTTVPCLCL LGPNLKAWLR VFSRNGVTSI
WENSLQITTI SSFVGAWLGA LPIPLDWERP WQVWPISCTL GATFGYVAGL
VISPLWIYWN RKQLTYKNN
UCSC uc002rvc.4; human. [Q07326-1];

Category

Human PIGF ELISA Kit

Human PIG-F ELISA Kit;Human GPI11 homolog ELISA Kit;Human phosphatidylinositol glycan anchor biosynthesis class F ELISA Kit;Human phosphatidylinositol-glycan biosynthesis class F protein ELISA Kit;