Intended UseMouse Ppia ELISA Kit allows for the in vitro quantitative determination of Ppia , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalFor samples:For the quantitative determination of mouse cyclophilin A (CyPA) concentrations in serum, plasma, cell culture supernates, tissue homogenates.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CyPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CyPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CyPA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CyPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Peptidyl-prolyl cis-trans isomerase A, N-terminally processed Protein information
P17742; Q9CWJ5; Q9R137;
Mouse Peptidyl-prolyl cis-trans isomerase A, N-terminally processed Protein SEQUENCE