Human FCGR2B ELISA Kit

Alternative name
Human igG Fc receptor II-b ELISA Kit;Human CDw32 ELISA Kit;Human fc-gamma RII-b ELISA Kit;Human fc-gamma-RIIb ELISA Kit;Human fcRII-b ELISA Kit;Human CD32 ELISA Kit;Human FCG2 ELISA Kit;Human IGFR2 ELISA Kit;Human CD32B ELISA Kit;Human FCGR2 ELISA Kit;Human Fc fragment of IgG receptor IIb ELISA Kit;Human low affinity immunoglobulin gamma Fc region receptor II-b ELISA Kit;Human Fc fragment of IgG, low affinity II, receptor for (CD32) ELISA Kit;Human Fc fragment of IgG, low affinity IIb, receptor (CD32) ELISA Kit;Human Fc fragment of IgG, low affinity IIb, receptor for (CD32) ELISA Kit;Human Fc gamma RIIb ELISA Kit;Human Fc gamma receptor IIb ELISA Kit;
Catalog
E007428
species
Human
GeneFCGR2B
SpecificityNatural and recombinant human CD32
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 1pg/ml
Intended UseHuman FCGR2B ELISA Kit allows for the in vitro quantitative determination of FCGR2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human CD32 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD32 has been precoated onto 96-well plates. Standards(NSO, A46-P217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD32 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD32 amount of sample captured in plate. Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
Human Low affinity immunoglobulin gamma Fc region receptor II-b Protein information
Uniprot ID FCG2B_HUMAN
Uniprot AC P31994; A6H8N3; O95649; Q53X85; Q5VXA9; Q8NIA1;
UniGene Hs.654395;
GeneID 2213
KEGG hsa:2213;
Human Low affinity immunoglobulin gamma Fc region receptor II-b Protein SEQUENCE
SEQUENCE 310 AA; 34044 MW; 2186F8538FF01F36 CRC64;
MGILSFLPVL ATESDWADCK SPQPWGHMLL WTAVLFLAPV AGTPAAPPKA
VLKLEPQWIN VLQEDSVTLT CRGTHSPESD SIQWFHNGNL IPTHTQPSYR
FKANNNDSGE YTCQTGQTSL SDPVHLTVLS EWLVLQTPHL EFQEGETIVL
RCHSWKDKPL VKVTFFQNGK SKKFSRSDPN FSIPQANHSH SGDYHCTGNI
GYTLYSSKPV TITVQAPSSS PMGIIVAVVT GIAVAAIVAA VVALIYCRKK
RISALPGYPE CREMGETLPE KPANPTNPDE ADKVGAENTI TYSLLMHPDA
LEEPDDQNRI
UCSC uc001gaz.3; human. [P31994-1];

Category

Human FCGR2B ELISA Kit