Mouse Pax9 ELISA Kit

Alternative name
Mouse Pax-9 ELISA Kit;Mouse paired box 9 ELISA Kit;Mouse paired box protein Pax-9 ELISA Kit;Mouse paired box gene 9 ELISA Kit;
Catalog
E062858
species
Mouse
GenePax9
SpecificityThis assay has high sensitivity and excellent specificity for detection of PAX9. No significant cross-reactivity or interference between PAX9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PAX9 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Pax9 ELISA Kit allows for the in vitro quantitative determination of Pax9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: PAX9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAX9 antibody and an PAX9-HRP conjugate. The assay sample and buffer are incubated together with PAX9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAX9 concentration since PAX9 from samples and PAX9-HRP conjugate compete for the anti-PAX9 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAX9 from the sample, fewer sites are left to bind PAX9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAX9 concentration in each sample is interpolated from this standard curve.
Mouse Paired box protein Pax-9 Protein information
Uniprot ID PAX9_MOUSE
Uniprot AC P47242;
UniGene Mm.5035;
GeneID 18511
KEGG mmu:18511;
Mouse Paired box protein Pax-9 Protein SEQUENCE
SEQUENCE 342 AA; 36389 MW; A4FB714FE541A96C CRC64;
MEPAFGEVNQ LGGVFVNGRP LPNAIRLRIV ELAQLGIRPC DISRQLRVSH
GCVSKILARY NETGSILPGA IGGSKPRVTT PTVVKHIRTY KQRDPGIFAW
EIRDRLLADG VCDKYNVPSV SSISRILRNK IGNLAQQGHY DSYKQHQPAP
QPALPYNHIY SYPSPITAAA AKVPTPPGVP AIPGSVALPR TWPSSHSVTD
ILGIRSITDQ GVSDSSPYHS PKVEEWSSLG RNNFPAAAPH AVNGLEKGAL
EQEAKYGQAP NGLPAVSSFV SASSMAPYPT PAQVSPYMTY SAAPSGYVAG
HGWQHAGSTP LSPHNCDIPA SLAFKGMQAA REGSHSVTAS AL
UCSC uc007npi.1; mouse.;

Category

Mouse Pax9 ELISA Kit

Mouse Pax-9 ELISA Kit;Mouse paired box 9 ELISA Kit;Mouse paired box protein Pax-9 ELISA Kit;Mouse paired box gene 9 ELISA Kit;