-
Alternative name
Putative macrophage stimulating 1-like protein ELISA KIT; Brain rescue factor 1 ELISA KIT; BRF-1 ELISA KIT; Hepatocyte growth factor-like protein homologMST1L ELISA KIT; D1F15S1A ELISA KIT; MST1P9 ELISA KIT; MSTP9 ELISA KIT; BRF-1 ELISA KIT
-
Catalog
E032394
-
species
Human
-
GeneMST1P9
-
SpecificityThis assay recognizes recombinant and natural human Putative macrophage-stimulating protein MSTP9. No significant cross-reactivity or interference was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Intended UseHuman MST1P9 ELISA Kit allows for the in vitro quantitative determination of MST1P9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Putative macrophage-stimulating protein MSTP9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Putative macrophage-stimulating protein MSTP9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Putative macrophage-stimulating protein MSTP9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Putative macrophage-stimulating protein MSTP9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-