Human SEPT3 ELISA Kit

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  • Alternative name

    Human SEP3 ELISA Kit;Human bK250D10.3 ELISA Kit;Human septin 3 ELISA Kit;Human neuronal-specific septin-3 ELISA Kit;

  • Catalog
    E034667
  • species
    Human
  • GeneSEPT3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Septin 3 (SEPT3). No significant cross-reactivity or interference between Septin 3 (SEPT3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.123ng/mL.
  • Intended UseHuman SEPT3 ELISA Kit allows for the in vitro quantitative determination of SEPT3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Septin 3 (SEPT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Septin 3 (SEPT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Septin 3 (SEPT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Septin 3 (SEPT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neuronal-specific septin-3 Protein information
  • Uniprot ID SEPT3_HUMAN
  • Uniprot AC Q9UH03; B1AHR0; Q2NKJ7; Q59GF7; Q6IBZ6; Q8N3P3; Q9HD35;
  • UniGene Hs.120483;
  • GeneID 55964
  • KEGG hsa:55964;
  • Human Neuronal-specific septin-3 Protein SEQUENCE
  • SEQUENCE 358 AA; 40704 MW; B49B0D1FBD9DE43B CRC64;

    MSKGLPETRT DAAMSELVPE PRPKPAVPMK PMSINSNLLG YIGIDTIIEQ

    MRKKTMKTGF DFNIMVVGQS GLGKSTLVNT LFKSQVSRKA SSWNREEKIP

    KTVEIKAIGH VIEEGGVKMK LTVIDTPGFG DQINNENCWE PIEKYINEQY

    EKFLKEEVNI ARKKRIPDTR VHCCLYFISP TGHSLRPLDL EFMKHLSKVV

    NIIPVIAKAD TMTLEEKSEF KQRVRKELEV NGIEFYPQKE FDEDLEDKTE

    NDKIRQESMP FAVVGSDKEY QVNGKRVLGR KTPWGIIEVE NLNHCEFALL

    RDFVIRTHLQ DLKEVTHNIH YETYRAKRLN DNGGLPPGEG LLGTVLPPVP

    ATPCPTAE

  • UCSC uc003bbr.5; human. [Q9UH03-1];



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