Human RNASE8 ELISA Kit

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  • Alternative name

    Human RNase 8 ELISA Kit;Human RAE2 ELISA Kit;Human ribonuclease A family member 8 ELISA Kit;Human ribonuclease 8 ELISA Kit;Human RNase 8, placental ELISA Kit;Human ribonuclease, RNase A family, 8 ELISA Kit;

  • Catalog
    E033606
  • species
    Human
  • GeneRNASE8
  • Standard CurveHuman RNASE8 ELISA Kit
  • Other Species Mouse RNASE8 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Ribonuclease A8 (RNASE8). No significant cross-reactivity or interference between Ribonuclease A8 (RNASE8) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.109ng/mL.
  • Intended UseHuman RNASE8 ELISA Kit allows for the in vitro quantitative determination of RNASE8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A8 (RNASE8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A8 (RNASE8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A8 (RNASE8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease A8 (RNASE8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Ribonuclease 8 Protein information
  • Uniprot ID RNAS8_HUMAN
  • Uniprot AC Q8TDE3; B2RPP6; B2RPP7;
  • UniGene Hs.553572;
  • GeneID 122665
  • KEGG hsa:122665;
  • Human Ribonuclease 8 Protein SEQUENCE
  • SEQUENCE 154 AA; 17041 MW; 25E6AA09B869DC8F CRC64;

    MAPARAGCCP LLLLLLGLWV AEVLVRAKPK DMTSSQWFKT QHVQPSPQAC

    NSAMSIINKY TERCKDLNTF LHEPFSSVAI TCQTPNIACK NSCKNCHQSH

    GPMSLTMGEL TSGKYPNCRY KEKHLNTPYI VACDPPQQGD PGYPLVPVHL

    DKVV

  • UCSC uc010tlm.3; human.;



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