Mouse Crkl ELISA Kit

Alternative name
Mouse Crkol ELISA Kit;Mouse 1110025F07Rik ELISA Kit;Mouse AA589403 ELISA Kit;Mouse AI325100 ELISA Kit;Mouse v-crk avian sarcoma virus CT10 oncogene homolog-like ELISA Kit;Mouse crk-like protein ELISA Kit;Mouse v-crk sarcoma virus CT10 oncogene homolog-like ELISA Kit;
Catalog
E049673
species
Mouse
GeneCrkl
Standard Curve
Other Species Human CRKL ELISA Kit Rat Crkl ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CRKL. No significant cross-reactivity or interference between CRKL and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse CRKL is typically less than 33pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding
Intended UseMouse Crkl ELISA Kit allows for the in vitro quantitative determination of Crkl , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRKL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CRKL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CRKL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Crk-like protein Protein information
Uniprot ID CRKL_MOUSE
Uniprot AC P47941; Q3TQ18; Q8BGC5;
UniGene Mm.21048; Mm.451241; Mm.486420;
GeneID 12929
KEGG mmu:12929;
Mouse Crk-like protein Protein SEQUENCE
SEQUENCE 303 AA; 33830 MW; A8C801F78EF73573 CRC64;
MSSARFDSSD RSAWYMGPVT RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL
SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE
PAPRYPSPPV GSVSAPNLPT AEENLEYVRT LYDFPGNDAE DLPFKKGELL
VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI
PEPAHAYAQP QTTTPLPTVA STPGAAINPL PSTQNGPVFA KAIQKRVPCA
YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD
DNE
UCSC uc007ykv.2; mouse.;

Category

Mouse Crkl ELISA Kit

Mouse Crkol ELISA Kit;Mouse 1110025F07Rik ELISA Kit;Mouse AA589403 ELISA Kit;Mouse AI325100 ELISA Kit;Mouse v-crk avian sarcoma virus CT10 oncogene homolog-like ELISA Kit;Mouse crk-like protein ELISA Kit;Mouse v-crk sarcoma virus CT10 oncogene homolog-like ELISA Kit;