Intended UseHuman Tb4 ELISA Kit allows for the in vitro quantitative determination of Tb4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of Tb4 in human serum, plasma and other biological fluids.
Principle of the Assay||This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tb4 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tb4 and unlabeled Tb4 (Standards or samples) with the pre-coated antibody specific to Tb4. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tb4 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tb4 in the sample.