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Alternative name
C-C motif chemokine 19 ELISA KIT; Beta-chemokine exodus-3 ELISA KIT; CK beta-11 ELISA KIT; Epstein-Barr virus-induced molecule 1 ligand chemokine ELISA KIT; EBI1 ligand chemokine ELISA KIT; ELC ELISA KIT; Macrophage inflammatory protein 3 beta ELISA KIT; MIP-3-beta ELISA KIT; Small-inducible cytokine A19CCL19 ELISA KIT; ELC ELISA KIT; MIP3B ELISA KIT; SCYA19 ELISA KIT; EBI1 ligand chemokine ELISA KIT; ELC ELISA KIT; MIP-3-beta ELISA KIT
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Catalog
E059280
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species
Mouse
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GeneMIP3b
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SpecificityThis assay has high sensitivity and excellent specificity for detection of MIP3b. No significant cross-reactivity or interference between MIP3b and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse MIP3b is typically less than 5.9pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
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Intended UseMouse MIP3b ELISA Kit allows for the in vitro quantitative determination of MIP3b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MIP3b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MIP3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MIP3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MIP3b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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