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Catalog
E030645
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species
Human
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GeneProGRP
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Pro-Gastrin Releasing Peptide (ProGRP).
No significant cross-reactivity or interference between Pro-Gastrin Releasing Peptide (ProGRP) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 4.31pg/mL.
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Intended UseHuman ProGRP ELISA Kit allows for the in vitro quantitative determination of ProGRP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pro-Gastrin Releasing Peptide (ProGRP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pro-Gastrin Releasing Peptide (ProGRP) and unlabeled Pro-Gastrin Releasing Peptide (ProGRP) (Standards or samples) with the pre-coated antibody specific to Pro-Gastrin Releasing Peptide (ProGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pro-Gastrin Releasing Peptide (ProGRP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pro-Gastrin Releasing Peptide (ProGRP) in the sample.
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