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Mouse Bace1 ELISA Kit

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  • Alternative name

    Mouse aspartyl protease 2 ELISA Kit;Mouse ASP2 ELISA Kit;Mouse asp 2 ELISA Kit;Mouse beta-site amyloid precursor protein cleaving enzyme 1 ELISA Kit;Mouse beta-site APP cleaving enzyme 1 ELISA Kit;Mouse memapsin-2 ELISA Kit;Mouse membrane-associated aspartic protease 2 ELISA Kit;Mouse Bace ELISA Kit;Mouse C76936 ELISA Kit;Mouse beta-secretase 1 ELISA Kit;Mouse APP beta-secretase ELISA Kit;
  • Catalog
    E045422
  • species
    Mouse
  • GeneBace1
  • Other Species Human BACE1 ELISA KitBovine BACE1 ELISA KitGuinea Pig BACE1 ELISA KitRat Bace1 ELISA Kit
  • SpecificityThis kit recognizes natural and recombinant Mouse BACE1. No significant cross-reactivity or interference between Mouse BACE1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Mouse BACE1 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseMouse Bace1 ELISA Kit allows for the in vitro quantitative determination of Bace1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BACE1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BACE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of BACE1. You can calculate the concentration of BACE1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Beta-secretase 1 Protein information
  • Uniprot ID BACE1_MOUSE
  • Uniprot AC P56818; Q544D0;
  • UniGene Mm.24044;
  • GeneID 23821
  • KEGG mmu:23821;
  • Mouse Beta-secretase 1 Protein SEQUENCE

  • SEQUENCE 501 AA; 55748 MW; C085A013145E474E CRC64;

    MAPALHWLLL WVGSGMLPAQ GTHLGIRLPL RSGLAGPPLG LRLPRETDEE

    SEEPGRRGSF VEMVDNLRGK SGQGYYVEMT VGSPPQTLNI LVDTGSSNFA

    VGAAPHPFLH RYYQRQLSST YRDLRKGVYV PYTQGKWEGE LGTDLVSIPH

    GPNVTVRANI AAITESDKFF INGSNWEGIL GLAYAEIARP DDSLEPFFDS

    LVKQTHIPNI FSLQLCGAGF PLNQTEALAS VGGSMIIGGI DHSLYTGSLW

    YTPIRREWYY EVIIVRVEIN GQDLKMDCKE YNYDKSIVDS GTTNLRLPKK

    VFEAAVKSIK AASSTEKFPD GFWLGEQLVC WQAGTTPWNI FPVISLYLMG

    EVTNQSFRIT ILPQQYLRPV EDVATSQDDC YKFAVSQSST GTVMGAVIME

    GFYVVFDRAR KRIGFAVSAC HVHDEFRTAA VEGPFVTADM EDCGYNIPQT

    DESTLMTIAY VMAAICALFM LPLCLMVCQW RCLRCLRHQH DDFADDISLL

    K

  • UCSC uc009pgh.2; mouse.;


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