Human LYPLA1 ELISA Kit

Alternative name
Human APT-1 ELISA Kit;Human hAPT1 ELISA Kit;Human Lysophospholipase 1 ELISA Kit;Human lysophospholipase I ELISA Kit;Human LPL-I ELISA Kit;Human lysoPLA I ELISA Kit;Human APT1 ELISA Kit;Human LPL1 ELISA Kit;Human acyl-protein thioesterase 1 ELISA Kit;Human lysophospholipid-specific lysophospholipase ELISA Kit;
Catalog
E000591
species
Human
GeneLYPLA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of LYPLA1. No significant cross-reactivity or interference between LYPLA1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LYPLA1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman LYPLA1 ELISA Kit allows for the in vitro quantitative determination of LYPLA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This LYPLA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LYPLA1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||LYPLA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LYPLA1 antibody and an LYPLA1-HRP conjugate. The assay sample and buffer are incubated together with LYPLA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LYPLA1 concentration since LYPLA1 from samples and LYPLA1-HRP conjugate compete for the anti-LYPLA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LYPLA1 from the sample, fewer sites are left to bind LYPLA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LYPLA1 concentration in each sample is interpolated from this standard curve.
Human Acyl-protein thioesterase 1 Protein information
Uniprot ID LYPA1_HUMAN
Uniprot AC O75608; O43202; Q9UQF9;
UniGene Hs.744046;
GeneID 10434
KEGG hsa:10434;
Human Acyl-protein thioesterase 1 Protein SEQUENCE
SEQUENCE 230 AA; 24670 MW; 90C0522F765F1AC6 CRC64;
MCGNNMSTPL PAIVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIRSSHIK
YICPHAPVRP VTLNMNVAMP SWFDIIGLSP DSQEDESGIK QAAENIKALI
DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA
SFPQGPIGGA NRDISILQCH GDCDPLVPLM FGSLTVEKLK TLVNPANVTF
KTYEGMMHSS CQQEMMDVKQ FIDKLLPPID
UCSC uc003xrz.5; human. [O75608-1];

Human LYPLA1 ELISA Kit

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