Mouse Flt3lg ELISA Kit

Alternative name
Mouse flt3 ligand ELISA Kit;Mouse Flt3l ELISA Kit;Mouse SL cytokine ELISA Kit;Mouse Flt3lg ELISA Kit;Mouse Ly72L ELISA Kit;Mouse FMS-like tyrosine kinase 3 ligand ELISA Kit;Mouse fms-related tyrosine kinase 3 ligand ELISA Kit;
Catalog
E053231
species
Mouse
GeneFlt3lg
SpecificityNatural and recombinant mouse Flt-3ligand
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Flt3lg ELISA Kit allows for the in vitro quantitative determination of Flt3lg , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Flt-3ligand has been precoated onto 96-well plates. Standards(NSO, G27-R188) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Flt-3ligand amount of sample captured in plate. Background: Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
Mouse Fms-related tyrosine kinase 3 ligand Protein information
Uniprot ID FLT3L_MOUSE
Uniprot AC P49772; Q64085;
UniGene Mm.1658;
GeneID 14256
KEGG mmu:14256;
Mouse Fms-related tyrosine kinase 3 ligand Protein SEQUENCE
SEQUENCE 232 AA; 26141 MW; 3A3680D3CB69FBA6 CRC64;
MTVLAPAWSP NSSLLLLLLL LSPCLRGTPD CYFSHSPISS NFKVKFRELT
DHLLKDYPVT VAVNLQDEKH CKALWSLFLA QRWIEQLKTV AGSKMQTLLE
DVNTEIHFVT SCTFQPLPEC LRFVQTNISH LLKDTCTQLL ALKPCIGKAC
QNFSRCLEVQ CQPDSSTLLP PRSPIALEAT ELPEPRPRQL LLLLLLLLPL
TLVLLAAAWG LRWQRARRRG ELHPGVPLPS HP
UCSC uc009gtn.2; mouse. [P49772-1];

Category

Mouse Flt3lg ELISA Kit

Mouse flt3 ligand ELISA Kit;Mouse Flt3l ELISA Kit;Mouse SL cytokine ELISA Kit;Mouse Flt3lg ELISA Kit;Mouse Ly72L ELISA Kit;Mouse FMS-like tyrosine kinase 3 ligand ELISA Kit;Mouse fms-related tyrosine kinase 3 ligand ELISA Kit;