Human F7 ELISA Kit

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  • Alternative name

    Human proconvertin ELISA Kit;Human Serum prothrombin conversion accelerator ELISA Kit;Human SPCA ELISA Kit;Human coagulation factor VII ELISA Kit;Human FVII coagulation protein ELISA Kit;Human coagulation factor VII (serum prothrombin conversion accelerator) ELISA Kit;Human eptacog alfa ELISA Kit;

  • Catalog
    E009832
  • species
    Human
  • GeneF7
  • Standard CurveHuman F7 ELISA Kit
  • Other Species Mouse F7 ELISA KitBovine F7 ELISA KitRabbit F7 ELISA KitRat F7 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Coagulation Factor VII (F7). No significant cross-reactivity or interference between Coagulation Factor VII (F7) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 19.56pg/mL.
  • Intended UseHuman F7 ELISA Kit allows for the in vitro quantitative determination of F7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VII (F7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor VII (F7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor VII (F7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor VII (F7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Factor VII heavy chain Protein information
  • Uniprot ID FA7_HUMAN
  • Uniprot AC P08709; B0YJC8; Q14339; Q5JVF1; Q5JVF2; Q9UD52; Q9UD53; Q9UD54;
  • UniGene Hs.36989;
  • GeneID 2155
  • KEGG hsa:2155;
  • Human Factor VII heavy chain Protein SEQUENCE
  • SEQUENCE 466 AA; 51594 MW; 9B5D501669D67B06 CRC64;

    MVSQALRLLC LLLGLQGCLA AGGVAKASGG ETRDMPWKPG PHRVFVTQEE

    AHGVLHRRRR ANAFLEELRP GSLERECKEE QCSFEEAREI FKDAERTKLF

    WISYSDGDQC ASSPCQNGGS CKDQLQSYIC FCLPAFEGRN CETHKDDQLI

    CVNENGGCEQ YCSDHTGTKR SCRCHEGYSL LADGVSCTPT VEYPCGKIPI

    LEKRNASKPQ GRIVGGKVCP KGECPWQVLL LVNGAQLCGG TLINTIWVVS

    AAHCFDKIKN WRNLIAVLGE HDLSEHDGDE QSRRVAQVII PSTYVPGTTN

    HDIALLRLHQ PVVLTDHVVP LCLPERTFSE RTLAFVRFSL VSGWGQLLDR

    GATALELMVL NVPRLMTQDC LQQSRKVGDS PNITEYMFCA GYSDGSKDSC

    KGDSGGPHAT HYRGTWYLTG IVSWGQGCAT VGHFGVYTRV SQYIEWLQKL

    MRSEPRPGVL LRAPFP

  • UCSC uc001vsv.5; human. [P08709-1];


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