Human DDIT3 ELISA Kit

Alternative name
Human DDIT-3 ELISA Kit;Human C/EBP zeta ELISA Kit;Human C/EBP-homologous protein ELISA Kit;Human CHOP ELISA Kit;Human c/EBP-homologous protein 10 ELISA Kit;Human CHOP-10 ELISA Kit;Human CCAAT/enhancer-binding protein homologous protein ELISA Kit;Human growth arrest and DNA damage-inducible protein GADD153 ELISA Kit;Human CHOP10 ELISA Kit;Human GADD153 ELISA Kit;Human CEBPZ ELISA Kit;Human DNA damage inducible transcript 3 ELISA Kit;Human DNA damage-inducible transcript 3 protein ELISA Kit;
Catalog
E012124
species
Human
GeneDDIT3
SpecificityThis assay has high sensitivity and excellent specificity for detection of DNA Damage Inducible Transcript 3 (DDIT3). No significant cross-reactivity or interference between DNA Damage Inducible Transcript 3 (DDIT3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.114ng/mL.
Intended UseHuman DDIT3 ELISA Kit allows for the in vitro quantitative determination of DDIT3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Damage Inducible Transcript 3 (DDIT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Damage Inducible Transcript 3 (DDIT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Damage Inducible Transcript 3 (DDIT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Damage Inducible Transcript 3 (DDIT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human DNA damage-inducible transcript 3 protein Protein information
Uniprot ID DDIT3_HUMAN
Uniprot AC P35638; F8VS99;
UniGene Hs.505777;
GeneID 1649
KEGG hsa:1649;
Human DNA damage-inducible transcript 3 protein Protein SEQUENCE
SEQUENCE 169 AA; 19175 MW; 31905293FB1FBBE2 CRC64;
MAAESLPFSF GTLSSWELEA WYEDLQEVLS SDENGGTYVS PPGNEEEESK
IFTTLDPASL AWLTEEEPEP AEVTSTSQSP HSPDSSQSSL AQEEEEEDQG
RTRKRKQSGH SPARAGKQRM KEKEQENERK VAQLAEENER LKQEIERLTR
EVEATRRALI DRMVNLHQA
UCSC uc009zps.4; human. [P35638-1];

Human DDIT3 ELISA Kit

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