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Human CDH19 ELISA Kit

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  • Alternative name

    Human CDH7L2 ELISA Kit;Human CDH7 ELISA Kit;Human cadherin 19 ELISA Kit;Human cadherin-19 ELISA Kit;Human cadherin 19, type 2 ELISA Kit;
  • Catalog
    E005846
  • species
    Human
  • GeneCDH19
  • Standard CurveHuman CDH19 ELISA Kit
  • Other Species Mouse CDH19 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CDH19. No significant cross-reactivity or interference between CDH19 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDH19 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CDH19 ELISA Kit allows for the in vitro quantitative determination of CDH19 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the assay: CDH19 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDH19 antibody and an CDH19-HRP conjugate. The assay sample and buffer are incubated together with CDH19-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDH19 concentration since CDH19 from samples and CDH19-HRP conjugate compete for the anti-CDH19 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDH19 from the sample, fewer sites are left to bind CDH19-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDH19 concentration in each sample is interpolated from this standard curve.
  • Human Cadherin-19 Protein information
  • Uniprot ID CAD19_HUMAN
  • Uniprot AC Q9H159; B4E1V5; F5H1K0;
  • UniGene Hs.42771;
  • GeneID 28513
  • KEGG hsa:28513;
  • Human Cadherin-19 Protein SEQUENCE

  • SEQUENCE 772 AA; 87002 MW; 650AD27480343C39 CRC64;

    MNCYLLLRFM LGIPLLWPCL GATENSQTKK VKQPVRSHLR VKRGWVWNQF

    FVPEEMNTTS HHIGQLRSDL DNGNNSFQYK LLGAGAGSTF IIDERTGDIY

    AIQKLDREER SLYILRAQVI DIATGRAVEP ESEFVIKVSD INDNEPKFLD

    EPYEAIVPEM SPEGTLVIQV TASDADDPSS GNNARLLYSL LQGQPYFSVE

    PTTGVIRISS KMDRELQDEY WVIIQAKDMI GQPGALSGTT SVLIKLSDVN

    DNKPIFKESL YRLTVSESAP TGTSIGTIMA YDNDIGENAE MDYSIEEDDS

    QTFDIITNHE TQEGIVILKK KVDFEHQNHY GIRAKVKNHH VPEQLMKYHT

    EASTTFIKIQ VEDVDEPPLF LLPYYVFEVF EETPQGSFVG VVSATDPDNR

    KSPIRYSITR SKVFNINDNG TITTSNSLDR EISAWYNLSI TATEKYNIEQ

    ISSIPLYVQV LNINDHAPEF SQYYETYVCE NAGSGQVIQT ISAVDRDESI

    EEHHFYFNLS VEDTNNSSFT IIDNQDNTAV ILTNRTGFNL QEEPVFYISI

    LIADNGIPSL TSTNTLTIHV CDCGDSGSTQ TCQYQELVLS MGFKTEVIIA

    ILICIMIIFG FIFLTLGLKQ RRKQILFPEK SEDFRENIFQ YDDEGGGEED

    TEAFDIAELR SSTIMRERKT RKTTSAEIRS LYRQSLQVGP DSAIFRKFIL

    EKLEEANTDP CAPPFDSLQT YAFEGTGSLA GSLSSLESAV SDQDESYDYL

    NELGPRFKRL ACMFGSAVQS NN

  • UCSC uc002lkc.3; human. [Q9H159-1];



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