Human CST4 ELISA Kit

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  • Alternative name

    Human Cystatin-4 ELISA Kit;Human cystatin-SA-III ELISA Kit;Human salivary acidic protein 1 ELISA Kit;Human cystatin S ELISA Kit;Human cystatin-S ELISA Kit;Human cystatin 4 ELISA Kit;

  • Catalog
    E010742
  • species
    Human
  • GeneCST4
  • Standard CurveHuman CST4 ELISA Kit
  • Other Species Mouse CST4 ELISA KitRat Cst4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cystatin 4 (CST4). No significant cross-reactivity or interference between Cystatin 4 (CST4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.053ng/mL.
  • Intended UseHuman CST4 ELISA Kit allows for the in vitro quantitative determination of CST4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin 4 (CST4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystatin 4 (CST4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin 4 (CST4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cystatin 4 (CST4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cystatin-S Protein information
  • Uniprot ID CYTS_HUMAN
  • Uniprot AC P01036; Q9UBI5; Q9UCS9;
  • UniGene Hs.654549;
  • GeneID 1472
  • KEGG hsa:1472;
  • Human Cystatin-S Protein SEQUENCE
  • SEQUENCE 141 AA; 16214 MW; 65B1FEB8F074DEA6 CRC64;

    MARPLCTLLL LMATLAGALA SSSKEENRII PGGIYDADLN DEWVQRALHF

    AISEYNKATE DEYYRRPLQV LRAREQTFGG VNYFFDVEVG RTICTKSQPN

    LDTCAFHEQP ELQKKQLCSF EIYEVPWEDR MSLVNSRCQE A

  • UCSC uc002wto.2; human.;



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