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Alternative name
Kallikrein-13 ELISA KIT; Kallikrein-like protein 4 ELISA KIT; KLK-L4KLK13 ELISA KIT; KLKL4 ELISA KIT; KLK-L4 ELISA KIT
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Catalog
E057702
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species
Mouse
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GeneKLK13
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Kallikrein 13 (KLK13).
No significant cross-reactivity or interference between Kallikrein 13 (KLK13) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 6.0pg/mL.
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Intended UseMouse KLK13 ELISA Kit allows for the in vitro quantitative determination of KLK13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 13 (KLK13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 13 (KLK13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 13 (KLK13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kallikrein 13 (KLK13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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