SpecificityThis assay has high sensitivity and excellent specificity for detection of 1,25(OH)2D. No significant cross-reactivity or interference between 1,25(OH)2D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 1,25(OH)2D and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman 1, 25 (OH) 2D3 ELISA Kit allows for the in vitro quantitative determination of 1, 25 (OH) 2D3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Uses: This 1,25(OH)2D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 1,25(OH)2D. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||1,25(OH)2D ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-1,25(OH)2D antibody and an 1,25(OH)2D-HRP conjugate. The assay sample and buffer are incubated together with 1,25(OH)2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 1,25(OH)2D concentration since 1,25(OH)2D from samples and 1,25(OH)2D-HRP conjugate compete for the anti-1,25(OH)2D antibody binding site. Since the number of sites is limited, as more sites are occupied by 1,25(OH)2D from the sample, fewer sites are left to bind 1,25(OH)2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 1,25(OH)2D concentration in each sample is interpolated from this standard curve.