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Alternative name
Ribosomal protein S6 kinase beta-1 ELISA KIT; 70 kDa ribosomal protein S6 kinase 1 ELISA KIT; P70S6K1 ELISA KIT; p70-S6K 1 ELISA KIT; Ribosomal protein S6 kinase I ELISA KIT; S6K ELISA KIT; p70 ribosomal S6 kinase alpha ELISA KIT; p70 S6 kinase alpha ELISA KIT; p70 S6K-alpha ELISA KIT; p70 S6KARps6kb1 ELISA KIT; S6K-beta-1 ELISA KIT; S6K1 ELISA KIT; P70S6K1 ELISA KIT; p70-S6K 1 ELISA KIT; S6K ELISA KIT; p70 S6 kinase alpha ELISA KIT; p70 S6K-alpha ELISA KIT; p70 S6KA ELISA KIT
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Catalog
E033706
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species
Human
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GeneRPS6Kbeta1
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<0.094ng/ml
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Intended UseHuman RPS6Kbeta1 ELISA Kit allows for the in vitro quantitative determination of RPS6Kbeta1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human RPS6Kbeta1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RPS6Kbeta1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RPS6Kbeta1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RPS6Kbeta1 can be calculated.
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