Human CASP3 ELISA Kit

Alternative name
Human CASP-3 ELISA Kit;Human apopain ELISA Kit;Human cysteine protease CPP32 ELISA Kit;Human CPP-32 ELISA Kit;Human protein Yama ELISA Kit;Human SREBP cleavage activity 1 ELISA Kit;Human SCA-1 ELISA Kit;Human CPP32 ELISA Kit;Human CPP32B ELISA Kit;Human caspase 3 ELISA Kit;Human caspase-3 ELISA Kit;Human PARP cleavage protease ELISA Kit;Human caspase 3, apoptosis-related cysteine peptidase ELISA Kit;Human caspase 3, apoptosis-related cysteine protease ELISA Kit;Human procaspase3 ELISA Kit;
Catalog
E006861
species
Human
GeneCASP3
Standard Curve
Other Species Mouse Casp3 ELISA Kit Bovine CASP3 ELISA Kit Canine CASP3 ELISA Kit Monkey CASP3 ELISA Kit Porcine CASP3 ELISA Kit Rabbit CASP3 ELISA Kit Rat Casp3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CASPASE-3. No significant cross-reactivity or interference between CASPASE-3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CASPASE-3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CASP3 ELISA Kit allows for the in vitro quantitative determination of CASP3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses||This CASPASE-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CASPASE-3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CASPASE-3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CASPASE-3. Standards or samples are then added to the microtiter plate wells and CASPASE-3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CASPASE-3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CASPASE-3 are added to each well to "sandwich? the CASPASE-3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CASPASE-3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASPASE-3 concentration in each sample is interpolated from this standard curve.
Human Caspase-3 subunit p12 Protein information
Uniprot ID CASP3_HUMAN
Uniprot AC P42574; A8K5M2; D3DP53; Q96AN1; Q96KP2;
UniGene Hs.141125;
GeneID 836
KEGG hsa:836;
Human Caspase-3 subunit p12 Protein SEQUENCE
SEQUENCE 277 AA; 31608 MW; 2F35CD3BCF7FF64A CRC64;
MENTENSVDS KSIKNLEPKI IHGSESMDSG ISLDNSYKMD YPEMGLCIII
NNKNFHKSTG MTSRSGTDVD AANLRETFRN LKYEVRNKND LTREEIVELM
RDVSKEDHSK RSSFVCVLLS HGEEGIIFGT NGPVDLKKIT NFFRGDRCRS
LTGKPKLFII QACRGTELDC GIETDSGVDD DMACHKIPVE ADFLYAYSTA
PGYYSWRNSK DGSWFIQSLC AMLKQYADKL EFMHILTRVN RKVATEFESF
SFDATFHAKK QIPCIVSMLT KELYFYH
UCSC uc003iwh.3; human.;

Related products with Human CASP3 ELISA Kit

Category

Human CASP3 ELISA Kit