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Mouse Sost ELISA Kit

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  • Alternative name

    Mouse 5430411E23Rik ELISA Kit;Mouse sclerostin ELISA Kit;
  • Catalog
    E067055
  • species
    Mouse
  • GeneSost
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse SOST. No significant cross-reactivity or interference between mouse SOST and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse SOST is typically less than 23.4 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseMouse Sost ELISA Kit allows for the in vitro quantitative determination of Sost , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOST is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOST bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse Sclerostin Protein information
  • Uniprot ID SOST_MOUSE
  • Uniprot AC Q99P68; Q9D3L7;
  • UniGene Mm.265602;
  • GeneID 74499
  • KEGG mmu:74499;
  • Mouse Sclerostin Protein SEQUENCE

  • SEQUENCE 211 AA; 23443 MW; AEB094E358E34961 CRC64;

    MQPSLAPCLI CLLVHAAFCA VEGQGWQAFR NDATEVIPGL GEYPEPPPEN

    NQTMNRAENG GRPPHHPYDA KGVSEYSCRE LHYTRFLTDG PCRSAKPVTE

    LVCSGQCGPA RLLPNAIGRV KWWRPNGPDF RCIPDRYRAQ RVQLLCPGGA

    APRSRKVRLV ASCKCKRLTR FHNQSELKDF GPETARPQKG RKPRPGARGA

    KANQAELENA Y




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