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Alternative name
Interleukin-36 beta ELISA KIT; FIL1 eta ELISA KIT; Interleukin-1 eta ELISA KIT; IL-1 eta ELISA KIT; Interleukin-1 family member 8 ELISA KIT; IL-1F8 ELISA KIT; Interleukin-1 homolog 2 ELISA KIT; IL-1H2IL36B ELISA KIT; IL1F8 ELISA KIT; IL1H2 ELISA KIT; IL-1 eta ELISA KIT; IL-1F8 ELISA KIT; IL-1H2 ELISA KIT
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Catalog
E057119
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species
Mouse
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GeneIL1F8
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse IL1F8. No significant cross-reactivity or interference between mouse IL1F8 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse IL1F8 is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
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Intended UseMouse IL1F8 ELISA Kit allows for the in vitro quantitative determination of IL1F8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1F8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1F8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1F8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1F8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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