Human HMG1 ELISA Kit

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  • Alternative name

    High mobility group protein B1 ELISA KIT; High mobility group protein 1 ELISA KIT; HMG-1HMGB1 ELISA KIT; HMG1 ELISA KIT; HMG-1 ELISA KIT

  • Catalog
    E018621
  • species
    Human
  • GeneHMG1
  • Other Species Mouse HMG1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HMG1. No significant cross-reactivity or interference between HMG1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity29.4pg/mL.
  • Intended UseHuman HMG1 ELISA Kit allows for the in vitro quantitative determination of HMG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HMG1 in human serum, plasma and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMG1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HMG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HMG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HMG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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