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Human LIPA ELISA Kit

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  • Alternative name

    Human acid cholesteryl ester hydrolase ELISA Kit;Human LAL ELISA Kit;Human cholesteryl esterase ELISA Kit;Human Lipase A ELISA Kit;Human sterol esterase ELISA Kit;Human CESD ELISA Kit;Human lipase A, lysosomal acid type ELISA Kit;Human lysosomal acid lipase/cholesteryl ester hydrolase ELISA Kit;Human cholesterol ester hydrolase ELISA Kit;Human lipase A, lysosomal acid, cholesterol esterase ELISA Kit;Human lysosomal acid lipase ELISA Kit;
  • Catalog
    E022766
  • species
    Human
  • GeneLIPA
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human LIPA. No significant cross-reactivity or interference between human LIPA and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human LIPA is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman LIPA ELISA Kit allows for the in vitro quantitative determination of LIPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LIPA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIPA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIPA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIPA bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Lysosomal acid lipase/cholesteryl ester hydrolase Protein information
  • Uniprot ID LICH_HUMAN
  • Uniprot AC P38571; B2RBH5; D3DR29; Q16529; Q53H21; Q5T074; Q5T771; Q96EJ0;
  • UniGene Hs.643030;
  • GeneID 3988
  • KEGG hsa:3988;
  • Human Lysosomal acid lipase/cholesteryl ester hydrolase Protein SEQUENCE

  • SEQUENCE 399 AA; 45419 MW; AC75A7909DA9195A CRC64;

    MKMRFLGLVV CLVLWTLHSE GSGGKLTAVD PETNMNVSEI ISYWGFPSEE

    YLVETEDGYI LCLNRIPHGR KNHSDKGPKP VVFLQHGLLA DSSNWVTNLA

    NSSLGFILAD AGFDVWMGNS RGNTWSRKHK TLSVSQDEFW AFSYDEMAKY

    DLPASINFIL NKTGQEQVYY VGHSQGTTIG FIAFSQIPEL AKRIKMFFAL

    GPVASVAFCT SPMAKLGRLP DHLIKDLFGD KEFLPQSAFL KWLGTHVCTH

    VILKELCGNL CFLLCGFNER NLNMSRVDVY TTHSPAGTSV QNMLHWSQAV

    KFQKFQAFDW GSSAKNYFHY NQSYPPTYNV KDMLVPTAVW SGGHDWLADV

    YDVNILLTQI TNLVFHESIP EWEHLDFIWG LDAPWRLYNK IINLMRKYQ

  • UCSC uc001kga.6; human. [P38571-1];



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