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Catalog
E056453
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species
Mouse
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GeneIgG
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse IgG. No significant cross-reactivity or interference between mouse IgG and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse IgG is typically less than 29 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse IgG concentration that could be differentiated from zero. It was determined the mean
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Intended UseMouse IgG ELISA Kit allows for the in vitro quantitative determination of IgG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with mouse IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for mouse IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of mouse IgG in samples. The color development is stopped and the intensity of the color is measured.
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