Human EN2 ELISA Kit

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  • Alternative name

    Human homeobox protein en-2 ELISA Kit;Human hu-En-2 ELISA Kit;Human engrailed homeobox 2 ELISA Kit;Human homeobox protein engrailed-2 ELISA Kit;Human engrailed homolog 2 ELISA Kit;Human engrailed-2 ELISA Kit;

  • Catalog
    E013181
  • species
    Human
  • GeneEN2
  • Other Species Mouse En2 ELISA KitChicken EN2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of EN2. No significant cross-reactivity or interference between EN2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.118ng/mL
  • Intended UseHuman EN2 ELISA Kit allows for the in vitro quantitative determination of EN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EN2 in human tissue homogenates and other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Homeobox protein engrailed-2 Protein information
  • Uniprot ID HME2_HUMAN
  • Uniprot AC P19622; A4D252; Q549U3; Q9UD58;
  • UniGene Hs.134989;
  • GeneID 2020
  • KEGG hsa:2020;
  • Human Homeobox protein engrailed-2 Protein SEQUENCE
  • SEQUENCE 333 AA; 34211 MW; ACF5399E383D6257 CRC64;

    MEENDPKPGE AAAAVEGQRQ PESSPGGGSG GGGGSSPGEA DTGRRRALML

    PAVLQAPGNH QHPHRITNFF IDNILRPEFG RRKDAGTCCA GAGGGRGGGA

    GGEGGASGAE GGGGAGGSEQ LLGSGSREPR QNPPCAPGAG GPLPAAGSDS

    PGDGEGGSKT LSLHGGAKKG GDPGGPLDGS LKARGLGGGD LSVSSDSDSS

    QAGANLGAQP MLWPAWVYCT RYSDRPSSGP RSRKPKKKNP NKEDKRPRTA

    FTAEQLQRLK AEFQTNRYLT EQRRQSLAQE LSLNESQIKI WFQNKRAKIK

    KATGNKNTLA VHLMAQGLYN HSTTAKEGKS DSE

  • UCSC uc003wmb.3; human.;




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