Mouse Ids ELISA Kit

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  • Alternative name

    Mouse alpha-L-iduronate sulfate sulfatase ELISA Kit;Mouse AW214631 ELISA Kit;Mouse iduronate 2-sulfatase ELISA Kit;Mouse iduronate sulfatase ELISA Kit;

  • Catalog
    E056197
  • species
    Mouse
  • GeneIds
  • Standard CurveMouse Ids ELISA Kit
  • Other Species Human IDS ELISA Kit
  • SpecificityNatural and recombinant mouse IDS
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of mouse IDS in cell culture supernates, serum and plasma
  • Intended UseMouse Ids ELISA Kit allows for the in vitro quantitative determination of Ids , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Mouse IDS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IDS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: T36-P552) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IDS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IDS amount of sample captured in plate. Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
  • Mouse Iduronate 2-sulfatase Protein information
  • Uniprot ID IDS_MOUSE
  • Uniprot AC Q08890; Q32KI7; Q3TM30;
  • UniGene Mm.233083;
  • GeneID 15931
  • KEGG mmu:15931;
  • Mouse Iduronate 2-sulfatase Protein SEQUENCE
  • SEQUENCE 552 AA; 62186 MW; 3E3D819A823AFDE5 CRC64;

    MSPPPPPPIW RQLSFSLLLG SFCIALESAA QGNSATDALN ILLIIVDDLR

    PSLGCYGDKL VRSPNIDQLA SHSVLFQNAF AQQAVCAPSR VSFLTGRRPD

    TTRLYDFNSY WRVHSGNFST IPQYFKENGY VTMSVGKVFH PGISSNHSDD

    YPYSWSFPPY HPSSEKYENT KTCKGQDGKL HANLLCPVDV ADVPEGTLPD

    KQSTEEAIRL LEKMKTSASP FFLAVGYHKP HIPFRYPKEF QKLYPLENIT

    LAPDPHVPDS LPPVAYNPWM DIREREDVQA LNISVPYGPI PEDFQRKIRQ

    SYFASVSYLD TQVGHVLSAL DDLRLAHNTI IAFTSDHGWA LGEHGEWAKY

    SNFDVATRVP LMLYVPGRTA PLPAAGQKLF PYRDPFDPAS DWMDAGRHTE

    DLVELVSLFP TLAGLAGLPV PPRCPIPSFH VELCREGQNL QKHLQLHDLE

    EEPDLFGNPR ELIAYSQYPR PADFPQWNSD KPSLNDIKVM GYSIRTVDYR

    YTVWVGFDPS EFLANFSDIH AGELYFVDSD PLQDHNVYND SQHGGLLHSL

    RP

  • UCSC uc012hjl.1; mouse.;


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