Human DLAT ELISA Kit

Alternative name
Human 70 kDa mitochondrial autoantigen of primary biliary cirrhosis ELISA Kit;Human PBC ELISA Kit;Human dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex ELISA Kit;Human M2 antigen complex 70 kDa subunit ELISA Kit;Human pyruvate dehydrogenase complex component E2 ELISA Kit;Human PDC-E2 ELISA Kit;Human PDCE2 ELISA Kit;Human DLTA ELISA Kit;Human dihydrolipoamide S-acetyltransferase ELISA Kit;Human dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial ELISA Kit;Human E2 component of pyruvate dehydrogenase complex ELISA Kit;
Catalog
E012100
species
Human
GeneDLAT
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human DLAT. No significant cross-reactivity or interference between Human DLAT and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human DLAT and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman DLAT ELISA Kit allows for the in vitro quantitative determination of DLAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DLAT in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DLAT. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human DLAT and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DLAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human DLAT. You can calculate the concentration of Human DLAT in the samples by comparing the OD of the samples to the standard curve.
Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial Protein information
Uniprot ID ODP2_HUMAN
Uniprot AC P10515; Q16783; Q53EP3;
UniGene Hs.335551;
GeneID 1737
KEGG hsa:1737;
Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial Protein SEQUENCE
SEQUENCE 647 AA; 68997 MW; DD93A8E666E377C2 CRC64;
MWRVCARRAQ NVAPWAGLEA RWTALQEVPG TPRVTSRSGP APARRNSVTT
GYGGVRALCG WTPSSGATPR NRLLLQLLGS PGRRYYSLPP HQKVPLPSLS
PTMQAGTIAR WEKKEGDKIN EGDLIAEVET DKATVGFESL EECYMAKILV
AEGTRDVPIG AIICITVGKP EDIEAFKNYT LDSSAAPTPQ AAPAPTPAAT
ASPPTPSAQA PGSSYPPHMQ VLLPALSPTM TMGTVQRWEK KVGEKLSEGD
LLAEIETDKA TIGFEVQEEG YLAKILVPEG TRDVPLGTPL CIIVEKEADI
SAFADYRPTE VTDLKPQVPP PTPPPVAAVP PTPQPLAPTP SAPCPATPAG
PKGRVFVSPL AKKLAVEKGI DLTQVKGTGP DGRITKKDID SFVPSKVAPA
PAAVVPPTGP GMAPVPTGVF TDIPISNIRR VIAQRLMQSK QTIPHYYLSI
DVNMGEVLLV RKELNKILEG RSKISVNDFI IKASALACLK VPEANSSWMD
TVIRQNHVVD VSVAVSTPAG LITPIVFNAH IKGVETIAND VVSLATKARE
GKLQPHEFQG GTFTISNLGM FGIKNFSAII NPPQACILAI GASEDKLVPA
DNEKGFDVAS MMSVTLSCDH RVVDGAVGAQ WLAEFRKYLE KPITMLL

Category

Human DLAT ELISA Kit