Sensitivity Intended Uses For detection of MCP-2 in human serum, plasma, urine, tissue samples or cell
Intended UseHuman MCP-2 ELISA Kit allows for the in vitro quantitative determination of MCP-2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyCardiovascular; Immunology; Brain Disorders & Neuroscience; Cytokines & Cell Signalling; Bone & Mineral Metabolism; Research Assays; Assay Categories
Product Description specificalPrinciple of the assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-MCP-2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-MCP-2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MCP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MCP-2 can be calculated.
Background: Monocyte chemotactic protein 2 (MCP-2), also known as Chemokine (C-C motif) ligand 8 (CCL8), is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes. CCL8 is a small cytokine belonging to the CC chemokine family. It is a potent inhibitor of HIV-1 by virtue of its high-affinity binding to the receptor CCR5, one of the major co-receptors for HIV-1. It elicits its effects by binding to several different cell surface receptors called chemokine receptors. These receptors include CCR1, CCR2B and CCR5.