Mouse ADAM ELISA Kit

Contact Us
  • Alternative name

    A disintegrin and metalloproteinase with thrombospondin motifs 7 ELISA KIT; COMPaseAdamts7 ELISA KIT; ADAM-TS 7 ELISA KIT; ADAM-TS7 ELISA KIT; ADAMTS-7 ELISA KIT

  • Catalog
    E042229
  • species
    Mouse
  • GeneADAM
  • Standard CurveMouse ADAM ELISA Kit
  • Other Species Human ADAM ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse ADAM ELISA Kit allows for the in vitro quantitative determination of ADAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Principle of the Assay: ADAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM antibody and an ADAM-HRP conjugate. The assay sample and buffer are incubated together with ADAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM concentration since ADAM from samples and ADAM-HRP conjugate compete for the anti-ADAM antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM from the sample, fewer sites are left to bind ADAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM concentration in each sample is interpolated from this standard curve.



Inquiry About Mouse ADAM ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!