Mouse Hpse ELISA Kit

Alternative name
Mouse endo-glucoronidase ELISA Kit;Mouse Hpa ELISA Kit;Mouse HSE1 ELISA Kit;Mouse Hpr1 ELISA Kit;Mouse heparanase ELISA Kit;Mouse endoglycosidase heparanase ELISA Kit;
Catalog
E055494
species
Mouse
GeneHpse
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse HPSE. No significant cross-reactivity or interference between mouse HPSE and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse HPSE is typically less than 7.8 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseMouse Hpse ELISA Kit allows for the in vitro quantitative determination of Hpse , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HPSE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HPSE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HPSE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HPSE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Heparanase 50 kDa subunit Protein information
Uniprot ID HPSE_MOUSE
Uniprot AC Q6YGZ1; B2RS99; Q8K3K3;
UniGene Mm.265786;
GeneID 15442
KEGG mmu:15442;
Mouse Heparanase 50 kDa subunit Protein SEQUENCE
SEQUENCE 535 AA; 60066 MW; 6E73A8302FB8A0DF CRC64;
MLRLLLLWLW GPLGALAQGA PAGTAPTDDV VDLEFYTKRP LRSVSPSFLS
ITIDASLATD PRFLTFLGSP RLRALARGLS PAYLRFGGTK TDFLIFDPDK
EPTSEERSYW KSQVNHDICR SEPVSAAVLR KLQVEWPFQE LLLLREQYQK
EFKNSTYSRS SVDMLYSFAK CSGLDLIFGL NALLRTPDLR WNSSNAQLLL
DYCSSKGYNI SWELGNEPNS FWKKAHILID GLQLGEDFVE LHKLLQRSAF
QNAKLYGPDI GQPRGKTVKL LRSFLKAGGE VIDSLTWHHY YLNGRIATKE
DFLSSDVLDT FILSVQKILK VTKEITPGKK VWLGETSSAY GGGAPLLSNT
FAAGFMWLDK LGLSAQMGIE VVMRQVFFGA GNYHLVDENF EPLPDYWLSL
LFKKLVGPRV LLSRVKGPDR SKLRVYLHCT NVYHPRYQEG DLTLYVLNLH
NVTKHLKVPP PLFRKPVDTY LLKPSGPDGL LSKSVQLNGQ ILKMVDEQTL
PALTEKPLPA GSALSLPAFS YGFFVIRNAK IAACI
UCSC uc008yhw.1; mouse.;

Category

Mouse Hpse ELISA Kit

Mouse endo-glucoronidase ELISA Kit;Mouse Hpa ELISA Kit;Mouse HSE1 ELISA Kit;Mouse Hpr1 ELISA Kit;Mouse heparanase ELISA Kit;Mouse endoglycosidase heparanase ELISA Kit;