Human BTN1A1 ELISA Kit

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  • Alternative name

    Human BT ELISA Kit;Human BTN ELISA Kit;Human BTN1 ELISA Kit;Human butyrophilin subfamily 1 member A1 ELISA Kit;Human bK14H9.2 (butyrophilin, subfamily 1, member A1) ELISA Kit;

  • Catalog
    E005427
  • species
    Human
  • GeneBTN1A1
  • Standard CurveHuman BTN1A1 ELISA Kit
  • Other Species Mouse Btn1a1 ELISA KitBovine BTN1A1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Butyrophilin Subfamily 1, Member A1 (BTN1A1). No significant cross-reactivity or interference between Butyrophilin Subfamily 1, Member A1 (BTN1A1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman BTN1A1 ELISA Kit allows for the in vitro quantitative determination of BTN1A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Butyrophilin Subfamily 1, Member A1 (BTN1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Butyrophilin Subfamily 1, Member A1 (BTN1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Butyrophilin Subfamily 1, Member A1 (BTN1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Butyrophilin Subfamily 1, Member A1 (BTN1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Butyrophilin subfamily 1 member A1 Protein information
  • Uniprot ID BT1A1_HUMAN
  • Uniprot AC Q13410; Q4VAN3; Q4VAN4; Q9H458;
  • UniGene Hs.153058;
  • GeneID 696
  • KEGG hsa:696;
  • Human Butyrophilin subfamily 1 member A1 Protein SEQUENCE
  • SEQUENCE 526 AA; 58960 MW; 4585D5CE88A2ECA4 CRC64;

    MAVFPSSGLP RCLLTLILLQ LPKLDSAPFD VIGPPEPILA VVGEDAELPC

    RLSPNASAEH LELRWFRKKV SPAVLVHRDG REQEAEQMPE YRGRATLVQD

    GIAKGRVALR IRGVRVSDDG EYTCFFREDG SYEEALVHLK VAALGSDPHI

    SMQVQENGEI CLECTSVGWY PEPQVQWRTS KGEKFPSTSE SRNPDEEGLF

    TVAASVIIRD TSAKNVSCYI QNLLLGQEKK VEISIPASSL PRLTPWIVAV

    AVILMVLGLL TIGSIFFTWR LYNERPRERR NEFSSKERLL EELKWKKATL

    HAVDVTLDPD TAHPHLFLYE DSKSVRLEDS RQKLPEKTER FDSWPCVLGR

    ETFTSGRHYW EVEVGDRTDW AIGVCRENVM KKGFDPMTPE NGFWAVELYG

    NGYWALTPLR TPLPLAGPPR RVGIFLDYES GDISFYNMND GSDIYTFSNV

    TFSGPLRPFF CLWSSGKKPL TICPIADGPE RVTVIANAQD LSKEIPLSPM

    GEDSAPRDAD TLHSKLIPTQ PSQGAP

  • UCSC uc003nif.5; human.;




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