Human PLAA ELISA Kit

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  • Alternative name

    Human PLA2P ELISA Kit;Human PLAP ELISA Kit;Human DOA1 ELISA Kit;Human phospholipase A2 activating protein ELISA Kit;Human phospholipase A-2-activating protein ELISA Kit;Human DOA1 homolog ELISA Kit;

  • Catalog
    E029156
  • species
    Human
  • GenePLAA
  • Standard CurveHuman PLAA ELISA Kit
  • Other Species Mouse Plaa ELISA KitRat Plaa ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A2 Activating Protein (PLAA). No significant cross-reactivity or interference between Phospholipase A2 Activating Protein (PLAA) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.105ng/mL.
  • Intended UseHuman PLAA ELISA Kit allows for the in vitro quantitative determination of PLAA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2 Activating Protein (PLAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2 Activating Protein (PLAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2 Activating Protein (PLAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2 Activating Protein (PLAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Phospholipase A-2-activating protein Protein information
  • Uniprot ID PLAP_HUMAN
  • Uniprot AC Q9Y263; Q53EU5; Q5VY33; Q9NUL8; Q9NVE9; Q9UF53; Q9Y5L1;
  • UniGene Hs.27182;
  • GeneID 9373
  • KEGG hsa:9373;
  • Human Phospholipase A-2-activating protein Protein SEQUENCE
  • SEQUENCE 795 AA; 87157 MW; D6E7330AC9891637 CRC64;

    MTSGATRYRL SCSLRGHELD VRGLVCCAYP PGAFVSVSRD RTTRLWAPDS

    PNRSFTEMHC MSGHSNFVSC VCIIPSSDIY PHGLIATGGN DHNICIFSLD

    SPMPLYILKG HKNTVCSLSS GKFGTLLSGS WDTTAKVWLN DKCMMTLQGH

    TAAVWAVKIL PEQGLMLTGS ADKTVKLWKA GRCERTFSGH EDCVRGLAIL

    SETEFLSCAN DASIRRWQIT GECLEVYYGH TNYIYSISVF PNCRDFVTTA

    EDRSLRIWKH GECAQTIRLP AQSIWCCCVL DNGDIVVGAS DGIIRVFTES

    EDRTASAEEI KAFEKELSHA TIDSKTGDLG DINAEQLPGR EHLNEPGTRE

    GQTRLIRDGE KVEAYQWSVS EGRWIKIGDV VGSSGANQQT SGKVLYEGKE

    FDYVFSIDVN EGGPSYKLPY NTSDDPWLTA YNFLQKNDLN PMFLDQVAKF

    IIDNTKGQML GLGNPSFSDP FTGGGRYVPG SSGSSNTLPT ADPFTGAGRY

    VPGSASMGTT MAGVDPFTGN SAYRSAASKT MNIYFPKKEA VTFDQANPTQ

    ILGKLKELNG TAPEEKKLTE DDLILLEKIL SLICNSSSEK PTVQQLQILW

    KAINCPEDIV FPALDILRLS IKHPSVNENF CNEKEGAQFS SHLINLLNPK

    GKPANQLLAL RTFCNCFVGQ AGQKLMMSQR ESLMSHAIEL KSGSNKNIHI

    ALATLALNYS VCFHKDHNIE GKAQCLSLIS TILEVVQDLE ATFRLLVALG

    TLISDDSNAV QLAKSLGVDS QIKKYSSVSE PAKVSECCRF ILNLL

  • UCSC uc003zqd.4; human.;


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