Mouse Hspa12b ELISA Kit

Alternative name
Mouse 2700081N06Rik ELISA Kit;Mouse heat shock protein 12B ELISA Kit;Mouse heat shock 70 kDa protein 12B ELISA Kit;
Catalog
E055249
species
Mouse
GeneHspa12b
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse HSPA12B. No significant cross-reactivity or interference between mouse HSPA12B and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse HSPA12B is typically less than 7.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseMouse Hspa12b ELISA Kit allows for the in vitro quantitative determination of Hspa12b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSPA12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPA12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPA12B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPA12B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Heat shock 70 kDa protein 12B Protein information
Uniprot ID HS12B_MOUSE
Uniprot AC Q9CZJ2;
UniGene Mm.277465;
GeneID 72630
KEGG mmu:72630;
Mouse Heat shock 70 kDa protein 12B Protein SEQUENCE
SEQUENCE 685 AA; 76119 MW; 641FE753180EBE2B CRC64;
MLTVPEMGLQ GLYISSSPER SPVPSPPGSP RTQESCGIAP LTPSQSPKPE
ARALQQASFS VVVAIDFGTT SSGYAFSFAS DPEAIHMMRK WEGGDPGVAH
QKTPTCLLLT PEGIFHSFGY TARDYYHDLD PEEARDWLYF EKFKMKIHSA
TDLTLKTQLE AVNGKKMLAL EVFAHALRFF KEHALQELRE QSECMLEKGA
VRWVLTVPAI WKQPAKQFMR EAAYLAGLVS REDAEKLLIA LEPEAASVYC
RKLRLHQLMD LSSRTAGRGR LGERRSIDSS FRHAREQLRR SRHSRTFLVE
AGVGELWAEM QEGDRYMVAD CGGGTVDLTV HQLEQPHGTL KELYKASGGP
YGAVGVDLAF EQLLCRIFGE DFIAKFKRQR PAAWVDLTIA FEARKRTAGP
HRAGALNISL PFSFIDFYRK QRGHNVETAL RRSSVNLVKW SSQGMLRMSC
EAMNELFQPT VSGIIQHIEM LLAKPEVQGV KLLFLVGGFA ESAVLQHAVQ
EALGTRGLRV VVPHDVGLTI LKGAVLFGQA PGVVRVRRSP LTYGVGVLNR
FVPGHHPPEK LLVRDGRRWC TDVFERFVAA EQSVALGEEV RRSYCPARPG
QRRVLINLYC CAAEDARFIT DPGVRKCGAL SLELEPEGCP ENTGTSPSRR
EIRAAMQFGD TEIKVTAVDV STNRSVRAAI DFLSN
UCSC uc008mkr.1; mouse.;

Category

Mouse Hspa12b ELISA Kit

Mouse 2700081N06Rik ELISA Kit;Mouse heat shock protein 12B ELISA Kit;Mouse heat shock 70 kDa protein 12B ELISA Kit;