SpecificityThis assay has high sensitivity and excellent specificity for detection of PFR. No significant cross-reactivity or interference between PFR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PFR and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman FP ELISA Kit allows for the in vitro quantitative determination of FP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: PFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PFR antibody and an PFR-HRP conjugate. The assay sample and buffer are incubated together with PFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFR concentration since PFR from samples and PFR-HRP conjugate compete for the anti-PFR antibody binding site. Since the number of sites is limited, as more sites are occupied by PFR from the sample, fewer sites are left to bind PFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFR concentration in each sample is interpolated from this standard curve.