Human FcgammaRII/CD32 ELISA Kit

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  • Alternative name

    Fc gamma receptor RII ELISA KIT; Fc gamma receptor RII

  • Catalog
  • species
  • GeneFcgammaRII/CD32
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human FcgammaRII/CD32. No significant cross-reactivity or interference between Human FcgammaRII/CD32 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.39 ng/ml
  • Intended UseHuman FcgammaRII/CD32 ELISA Kit allows for the in vitro quantitative determination of FcgammaRII/CD32 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FcgammaRII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FcgammaRII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FcgammaRII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FcgammaRII bound in the initial step. The color development is stopped and the intensity of the color is measured.

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