SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse H2AFX. No significant cross-reactivity or interference between mouse H2AFX and analogues was observed.
SensitivityThe minimum detectable dose of mouse H2AFX is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseMouse H2afx ELISA Kit allows for the in vitro quantitative determination of H2afx , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for H2AFX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any H2AFX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for H2AFX is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of H2AFX bound in the initial step. The color development is stopped and the intensity of the color is measured.