Human RASSF1 ELISA Kit

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  • Alternative name

    Human 123F2 ELISA Kit;Human NORE2A ELISA Kit;Human RASSF1A ELISA Kit;Human RDA32 ELISA Kit;Human REH3P21 ELISA Kit;Human Ras association domain family member 1 ELISA Kit;Human ras association domain-containing protein 1 ELISA Kit;Human Ras association (RalGDS/AF-6) domain family member 1 ELISA Kit;Human WUGSC:H_LUCA12.5 ELISA Kit;Human cardiac-specific ras association domain family 1 protein ELISA Kit;Human pancreas-specific ras association domain family 1 protein ELISA Kit;Human tumor suppressor protein RDA32 ELISA Kit;

  • Catalog
    E032854
  • species
    Human
  • GeneRASSF1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RASSF1. No significant cross-reactivity or interference between RASSF1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.059ng/mL.
  • Intended UseHuman RASSF1 ELISA Kit allows for the in vitro quantitative determination of RASSF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RASSF1 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to RASSF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RASSF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RASSF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RASSF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Ras association domain-containing protein 1 Protein information
  • Uniprot ID RASF1_HUMAN
  • Uniprot AC Q9HB18; Q9NS22; Q9UND4; Q9UND5;
  • UniGene Hs.476270;
  • GeneID 11186
  • KEGG hsa:11186;
  • Human Ras association domain-containing protein 1 Protein SEQUENCE
  • SEQUENCE 344 AA; 39219 MW; 4071B8D044C1DB2D CRC64;

    MSGEPELIEL RELAPAGRAG KGRTRLERAN ALRIARGTAC NPTRQLVPGR

    GHRFQPAGPA THTWCDLCGD FIWGVVRKGL QCARLSADCK FTCHYRCRAL

    VCLDCCGPRD LGWEPAVERD TNVDEPVEWE TPDLSQAEIE QKIKEYNAQI

    NSNLFMSLNK DGSYTGFIKV QLKLVRPVSV PSSKKPPSLQ DARRGPGRGT

    SVRRRTSFYL PKDAVKHLHV LSRTRAREVI EALLRKFLVV DDPRKFALFE

    RAERHGQVYL RKLLDDEQPL RLRLLAGPSD KALSFVLKEN DSGEVNWDAF

    SMPELHNFLR ILQREEEEHL RQILQKYSYC RQKIQEALHA CPLG

  • UCSC uc003dab.2; human. [Q9NS23-1];



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