Mouse Ecm1 ELISA Kit

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  • Alternative name

    Mouse secretory component p85 ELISA Kit;Mouse AI663821 ELISA Kit;Mouse p85 ELISA Kit;Mouse extracellular matrix protein 1 ELISA Kit;

  • Catalog
    E051537
  • species
    Mouse
  • GeneEcm1
  • Other Species Human ECM1 ELISA KitRat Ecm1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse ECM1. No significant cross-reactivity or interference between Mouse ECM1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse ECM1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity2.813pg/mL
  • Intended UseMouse Ecm1 ELISA Kit allows for the in vitro quantitative determination of Ecm1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ECM1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ECM1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse ECM1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ECM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse ECM1. You can calculate the concentration of Mouse ECM1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Extracellular matrix protein 1 Protein information
  • Uniprot ID ECM1_MOUSE
  • Uniprot AC Q61508; Q3U5Q5;
  • UniGene Mm.3433;
  • GeneID 13601
  • KEGG mmu:13601;
  • Mouse Extracellular matrix protein 1 Protein SEQUENCE
  • SEQUENCE 559 AA; 62832 MW; F5282E5D7B78E696 CRC64;

    MGTVSRAALI LACLALASAA SEGAFKASDQ REMTPERLFQ HLHEVGYAAP

    PSPPQTRRLR VDHSVTSLHD PPLFEEQREV QPPSSPEDIP VYEEDWPTFL

    NPNVDKAGPA VPQEAIPLQK EQPPPQVHIE QKEIDPPAQP QEEIVQKEVK

    PHTLAGQLPP EPRTWNPARH CQQGRRGVWG HRLDGFPPGR PSPDNLKQIC

    LPERQHVIYG PWNLPQTGYS HLSRQGETLN VLETGYSRCC RCRSDTNRLD

    CLKLVWEDAM TQFCEAEFSV KTRPHLCCRL RGEERFSCFQ KEAPRPDYLL

    RPCPVHQNGM SSGPQLPFPP GLPTPDNVKN ICLLRRFRAV PRNLPATDAI

    QRQLQALTRL ETEFQRCCRQ GHNHTCTWKA WEGTLDGYCE RELAIKTHPH

    SCCHYPPSPA RDECFAHLAP YPNYDRDILT LDLSRVTPNL MGQLCGSGRV

    LSKHKQIPGL IQNMTIRCCE LPYPEQACCG EEEKLAFIEN LCGPRRNSWK

    DPALCCDLSP EDKQINCFNT NYLRNVALVA GDTGNATGLG EQGPTRGTDA

    NPAPGSKEE

  • UCSC uc008qko.2; mouse. [Q61508-1];



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