Mouse Grb2 ELISA Kit

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  • Alternative name

    Mouse adapter protein GRB2 ELISA Kit;Mouse SH2/SH3 adapter GRB2 ELISA Kit;Mouse AA408164 ELISA Kit;Mouse Ash ELISA Kit;Mouse growth factor receptor bound protein 2 ELISA Kit;Mouse growth factor receptor-bound protein 2 ELISA Kit;

  • Catalog
    E054829
  • species
    Mouse
  • GeneGrb2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse Grb2. No significant cross-reactivity or interference between Mouse Grb2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse Grb2 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Grb2 ELISA Kit allows for the in vitro quantitative determination of Grb2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Grb2 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Grb2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse Grb2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Grb2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse Grb2. You can calculate the concentration of Mouse Grb2 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Growth factor receptor-bound protein 2 Protein information
  • Uniprot ID GRB2_MOUSE
  • Uniprot AC Q60631; Q61240;
  • UniGene Mm.439649; Mm.490413;
  • GeneID 14784
  • KEGG mmu:14784;
  • Mouse Growth factor receptor-bound protein 2 Protein SEQUENCE
  • SEQUENCE 217 AA; 25238 MW; 97F4A4FE4B248DDF CRC64;

    MEAIAKYDFK ATADDELSFK RGDILKVLNE ECDQNWYKAE LNGKDGFIPK

    NYIEMKPHPW FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK

    FGNDVQHFKV LRDGAGKYFL WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD

    IEQMPQQPTY VQALFDFDPQ EDGELGFRRG DFIHVMDNSD PNWWKGACHG

    QTGMFPRNYV TPVNRNV

  • UCSC uc007mij.1; mouse. [Q60631-1];



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