SpecificityThe Mouse GITRL ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse GITRL proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Intended UseMouse Tnfsf18 ELISA Kit allows for the in vitro quantitative determination of Tnfsf18 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay||The Mouse GITRL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse GITRL in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse GITRL coated on a 96-well plate. Standards and samples are pipetted into the wells and GITRL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse GITRL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GITRL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Tumor necrosis factor ligand superfamily member 18, also known as AITRL or GITRL, is a protein that in humans is encoded by the TNFSF18 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. It is mapped to 1q25.1. TNFSF18 is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. TNFSF18-dependent modulation of tryptophan catabolism may represent an important mechanism of action of glucocorticoids, both physiologically and therapeutically.
Mouse Tumor necrosis factor ligand superfamily member 18 Protein information
Uniprot ID
TNF18_MOUSE
Uniprot AC
Q7TS55; Q7TNY2; Q80YG2;
UniGene
Mm.276823;
GeneID
240873
KEGG
mmu:240873;
Mouse Tumor necrosis factor ligand superfamily member 18 Protein SEQUENCE