Mouse Prss27 ELISA Kit

Alternative name
Mouse marapsin ELISA Kit;Mouse pancreasin ELISA Kit;Mouse Mpn ELISA Kit;Mouse CAPH2 ELISA Kit;Mouse protease, serine 27 ELISA Kit;Mouse serine protease 27 ELISA Kit;Mouse channel-activating protease 2-like protein ELISA Kit;
Catalog
E059504
species
Mouse
GenePrss27
SpecificityNatural and recombinant Mouse Marapsin/Pancresin
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
Intended UseMouse Prss27 ELISA Kit allows for the in vitro quantitative determination of Prss27 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: Mouse Marapsin/Pancresin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Marapsin/Pancresin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: M38-V328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Marapsin/Pancresin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Marapsin/Pancresin amount of sample captured in plate. Background/Introduction: Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.
Mouse Serine protease 27 Protein information
Uniprot ID PRS27_MOUSE
Uniprot AC Q8BJR6; Q14A25;
UniGene Mm.133083;
GeneID 213171
KEGG mmu:213171;
Mouse Serine protease 27 Protein SEQUENCE
SEQUENCE 328 AA; 35789 MW; DC0B20F1AB3EB840 CRC64;
MRQPHIAALL LLPLLLRSGT EGARTLRACG HPKMFNRMVG GENALEGEWP
WQVSIQRNGI HFCGGSLIAP TWVLTAAHCF SNTSDISIYQ VLLGALKLQQ
PGPHALYVPV KQVKSNPQYQ GMASSADVAL VELQGPVTFT NYILPVCLPD
PSVIFESGMN CWVTGWGSPS EQDRLPNPRV LQKLAVPIID TPKCNLLYNK
DVESDFQLKT IKDDMLCAGF AEGKKDACKG DSGGPLVCLV DQSWVQAGVI
SWGEGCARRN RPGVYIRVTS HHKWIHQIIP ELQFQGRAGT QQQQKDSQGQ
QRLAGNSAPC LAAHAMVLAL GALLLRIV
UCSC uc008aug.1; mouse.;

Category

Mouse Prss27 ELISA Kit

Mouse marapsin ELISA Kit;Mouse pancreasin ELISA Kit;Mouse Mpn ELISA Kit;Mouse CAPH2 ELISA Kit;Mouse protease, serine 27 ELISA Kit;Mouse serine protease 27 ELISA Kit;Mouse channel-activating protease 2-like protein ELISA Kit;