Human ACVR1B ELISA Kit

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  • Alternative name

    Human Activin receptor type IB ELISA Kit;Human ACTR-IB ELISA Kit;Human activin receptor-like kinase 4 ELISA Kit;Human ALK-4 ELISA Kit;Human serine/threonine-protein kinase receptor R2 ELISA Kit;Human SKR2 ELISA Kit;Human ACVRLK4 ELISA Kit;Human ALK4 ELISA Kit;Human ACTRIB ELISA Kit;Human activin A receptor type 1B ELISA Kit;Human activin receptor type-1B ELISA Kit;Human activin A receptor, type IB ELISA Kit;Human activin A receptor, type II-like kinase 4 ELISA Kit;

  • Catalog
    E000540
  • species
    Human
  • GeneACVR1B
  • Other Species Mouse Acvr1b ELISA KitRat Acvr1b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ACVR1B. No significant cross-reactivity or interference between ACVR1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACVR1B and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ACVR1B ELISA Kit allows for the in vitro quantitative determination of ACVR1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses||This ACVR1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACVR1B. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ACVR1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACVR1B antibody and an ACVR1B-HRP conjugate. The assay sample and buffer are incubated together with ACVR1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACVR1B concentration since ACVR1B from samples and ACVR1B-HRP conjugate compete for the anti-ACVR1B antibody binding site. Since the number of sites is limited, as more sites are occupied by ACVR1B from the sample, fewer sites are left to bind ACVR1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACVR1B concentration in each sample is interpolated from this standard curve.
  • Human Activin receptor type-1B Protein information
  • Uniprot ID ACV1B_HUMAN
  • Uniprot AC P36896; B7Z5L8; B7Z5W5; Q15479; Q15480; Q15481; Q15482;
  • UniGene Hs.438918;
  • GeneID 91
  • KEGG hsa:91;
  • Human Activin receptor type-1B Protein SEQUENCE
  • SEQUENCE 505 AA; 56807 MW; 40A6C65CAA4C7573 CRC64;

    MAESAGASSF FPLVVLLLAG SGGSGPRGVQ ALLCACTSCL QANYTCETDG

    ACMVSIFNLD GMEHHVRTCI PKVELVPAGK PFYCLSSEDL RNTHCCYTDY

    CNRIDLRVPS GHLKEPEHPS MWGPVELVGI IAGPVFLLFL IIIIVFLVIN

    YHQRVYHNRQ RLDMEDPSCE MCLSKDKTLQ DLVYDLSTSG SGSGLPLFVQ

    RTVARTIVLQ EIIGKGRFGE VWRGRWRGGD VAVKIFSSRE ERSWFREAEI

    YQTVMLRHEN ILGFIAADNK DNGTWTQLWL VSDYHEHGSL FDYLNRYTVT

    IEGMIKLALS AASGLAHLHM EIVGTQGKPG IAHRDLKSKN ILVKKNGMCA

    IADLGLAVRH DAVTDTIDIA PNQRVGTKRY MAPEVLDETI NMKHFDSFKC

    ADIYALGLVY WEIARRCNSG GVHEEYQLPY YDLVPSDPSI EEMRKVVCDQ

    KLRPNIPNWW QSYEALRVMG KMMRECWYAN GAARLTALRI KKTLSQLSVQ

    EDVKI

  • UCSC uc001rzl.4; human. [P36896-1];


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