Human ACVR1B ELISA Kit

Alternative name
Human Activin receptor type IB ELISA Kit;Human ACTR-IB ELISA Kit;Human activin receptor-like kinase 4 ELISA Kit;Human ALK-4 ELISA Kit;Human serine/threonine-protein kinase receptor R2 ELISA Kit;Human SKR2 ELISA Kit;Human ACVRLK4 ELISA Kit;Human ALK4 ELISA Kit;Human ACTRIB ELISA Kit;Human activin A receptor type 1B ELISA Kit;Human activin receptor type-1B ELISA Kit;Human activin A receptor, type IB ELISA Kit;Human activin A receptor, type II-like kinase 4 ELISA Kit;
Catalog
E000540
species
Human
GeneACVR1B
Other Species Mouse Acvr1b ELISA Kit Rat Acvr1b ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ACVR1B. No significant cross-reactivity or interference between ACVR1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACVR1B and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ACVR1B ELISA Kit allows for the in vitro quantitative determination of ACVR1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses||This ACVR1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACVR1B. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ACVR1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ACVR1B antibody and an ACVR1B-HRP conjugate. The assay sample and buffer are incubated together with ACVR1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACVR1B concentration since ACVR1B from samples and ACVR1B-HRP conjugate compete for the anti-ACVR1B antibody binding site. Since the number of sites is limited, as more sites are occupied by ACVR1B from the sample, fewer sites are left to bind ACVR1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACVR1B concentration in each sample is interpolated from this standard curve.
Human Activin receptor type-1B Protein information
Uniprot ID ACV1B_HUMAN
Uniprot AC P36896; B7Z5L8; B7Z5W5; Q15479; Q15480; Q15481; Q15482;
UniGene Hs.438918;
GeneID 91
KEGG hsa:91;
Human Activin receptor type-1B Protein SEQUENCE
SEQUENCE 505 AA; 56807 MW; 40A6C65CAA4C7573 CRC64;
MAESAGASSF FPLVVLLLAG SGGSGPRGVQ ALLCACTSCL QANYTCETDG
ACMVSIFNLD GMEHHVRTCI PKVELVPAGK PFYCLSSEDL RNTHCCYTDY
CNRIDLRVPS GHLKEPEHPS MWGPVELVGI IAGPVFLLFL IIIIVFLVIN
YHQRVYHNRQ RLDMEDPSCE MCLSKDKTLQ DLVYDLSTSG SGSGLPLFVQ
RTVARTIVLQ EIIGKGRFGE VWRGRWRGGD VAVKIFSSRE ERSWFREAEI
YQTVMLRHEN ILGFIAADNK DNGTWTQLWL VSDYHEHGSL FDYLNRYTVT
IEGMIKLALS AASGLAHLHM EIVGTQGKPG IAHRDLKSKN ILVKKNGMCA
IADLGLAVRH DAVTDTIDIA PNQRVGTKRY MAPEVLDETI NMKHFDSFKC
ADIYALGLVY WEIARRCNSG GVHEEYQLPY YDLVPSDPSI EEMRKVVCDQ
KLRPNIPNWW QSYEALRVMG KMMRECWYAN GAARLTALRI KKTLSQLSVQ
EDVKI
UCSC uc001rzl.4; human. [P36896-1];

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Human ACVR1B ELISA Kit