Human TYMS ELISA Kit

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  • Alternative name

    Human TS ELISA Kit;Human TSase ELISA Kit;Human HST422 ELISA Kit;Human TMS ELISA Kit;Human thymidylate synthetase ELISA Kit;Human thymidylate synthase ELISA Kit;

  • Catalog
    E038174
  • species
    Human
  • GeneTYMS
  • Standard CurveHuman TYMS ELISA Kit
  • Other Species Human TS/TYMS ELISA KitMouse Tyms ELISA KitBovine TYMS ELISA KitRat Tyms ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Thymidylate Synthetase (TYMS). No significant cross-reactivity or interference between Thymidylate Synthetase (TYMS) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.113ng/mL.
  • Intended UseHuman TYMS ELISA Kit allows for the in vitro quantitative determination of TYMS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidylate Synthetase (TYMS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidylate Synthetase (TYMS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidylate Synthetase (TYMS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymidylate Synthetase (TYMS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Thymidylate synthase Protein information
  • Uniprot ID TYSY_HUMAN
  • Uniprot AC P04818; Q8WYK3; Q8WYK4;
  • UniGene Hs.369762;
  • GeneID 7298
  • KEGG hsa:7298;
  • Human Thymidylate synthase Protein SEQUENCE
  • SEQUENCE 313 AA; 35716 MW; 148D377F19915B6A CRC64;

    MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR

    TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE

    LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME

    SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ

    FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH

    TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG

    YNPHPTIKME MAV

  • UCSC uc010dkb.2; human. [P04818-1];



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